[Inducing peripheral materno-fetal immuno-tolerance by blockade of costimulatory signal at early stage of gestation of the abortion-prone murine model].

Abstract

OBJECTIVE:

To study effect of blockade of costimulatory signal CD(80), CD(86) at the early stage of gestation on the maternal peripheral immuno-tolerance status to paternal antigen and pregnant outcome of murine abortion-prone model.

METHODS:

The experiments were performed in two groups, using CBA/J x BALB/c matings as the normal pregnancy model and CBA/J x DBA/2 matings as the abortion-prone model. The pregnant CBA/J mice which had mated with DBA/2 or BALB/c male mice respectively were injected intraperitoneally with purified rat isotype IgG or purified rat anti-mouse CD(80), CD(86) mAb at day 4 of gestation (time of implantation). The proliferation response of maternal splenic immuno-competent cells to paternal splenic cells as stimulator was analyzed by one way mixed lymphocyte reaction, and IL-2 production was assayed by ELISA to evaluate the immuno-tolerance of maternal peripheral immuno-competent cells to paternal antigen at day 9 of gestation. The embryo resorption rate was counted at day 14 of gestation.

RESULTS:

In the CBA/J x DBA/2 matings, anti-CD(80), CD(86) mAb administered at day 4 of gestation reduced significantly the embryo resorption rate, and induced a significantly lower proliferation response and IL-2 production by maternal splenic immuno-competent cells to paternal antigen compared with rat isotype IgG.

CONCLUSIONS:

In vivo blockade of costimulatory signal at day 4 of gestation can induce the immuno-tolerance of maternal peripheral immuno-competent cells to paternal antigen, thus leading the embryo resorption rate of the abortion-prone model to that of normal pregnancy model.

• Authors: Li-ping Jin, Da-jin Li, Ming-yan Wang, Xiao-yong Zhu, Ying Zhu, Yi Meng, Min-min Yuan
• Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi) Vol. 39 Issue 4 Pg. 234-7 (Apr 2004) ISSN: 0529-567X [Print] China
• PMID: 15130348 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• B7-1 Antigen
• B7-2 Antigen
• Cd86 protein, mouse
• Interleukin-2
• Membrane Glycoproteins

Topics

• Abortion, Spontaneous (immunology)
• Animals
• Antigens, CD (immunology)
• B7-1 Antigen (immunology)
• B7-2 Antigen
• Enzyme-Linked Immunosorbent Assay
• Female
• Immune Tolerance (immunology)
• Interleukin-2 (analysis)
• Male
• Maternal-Fetal Exchange (immunology)
• Membrane Glycoproteins (immunology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred CBA
• Mice, Inbred DBA
• Models, Animal
• Pregnancy
• Signal Transduction (immunology)