Influence of nitric oxide on microcirculation in pancreatic ischemia/reperfusion injury: an intravital microscopic study.

Abstract

Recently, protective effects of nitric oxide donors in pancreatic ischemia/reperfusion (IRI) injury have been described. Their role in post-ischemic microcirculation was previously not investigated. Ischemia reperfusion was induced in an isolated pancreatic tail segment in situ. Animals were randomized to four experimental groups (n=7 animals/group), the control group (CO) received saline as placebo. Treatment groups received either sodium nitroprusside (SN) 5 min before until 2 h after reperfusion, L-arginine (LA) 30 min before reperfusion until 2 h after reperfusion or sodium nitroprusside and L-arginine (SNLA) together. After induction of ischemia (2 h) post-ischemic microcirculation was observed for 2 h by intravital-fluorescence microscopy. Functional-capillary density (FCD), leukocyte adherence in post-capillary venules (LAV) and histological damage were analysed. After reperfusion FCD decreased in all groups (P<0.05). FCD was significantly restored in all groups with administration of nitric oxide donors after reperfusion (P<0.05) as compared to CO without significant difference between the individual nitric oxide donor groups. Leukocyte adherence was significantly increased 1 h and 2 h after reperfusion (P<0.001) as compared to baseline, which was lower in all nitric oxide donor groups. Histological damage in the pancreatic tail-segment was significantly reduced in nitric oxide donor groups (P<0.01). Administration of nitric oxide donors might be useful in ischemia-reperfusion injury of the pancreas by its protective effect on microcirculation and inflammatory reaction.

• Authors: R Obermaier, E von Dobschuetz, O Muhs, T Keck, O Drognitz, L Jonas, W Schareck, U T Hopt, S Benz
• Journal: Transplant international : official journal of the European Society for Organ Transplantation (Transpl Int) Vol. 17 Issue 4 Pg. 208-14 (May 2004) ISSN: 0934-0874 [Print] Switzerland
• PMID: 15118815 (Publication Type: Journal Article)

Chemical References

• Nitric Oxide

Topics

• Animals
• Endothelium, Vascular (drug effects, physiopathology)
• Male
• Microcirculation (drug effects)
• Microscopy, Fluorescence
• Nitric Oxide (pharmacology)
• Pancreas (blood supply, drug effects)
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, physiopathology)