Abstract | BACKGROUND: METHODS: RESULTS: Twenty-eight of the 35 patients showed IIF-positive blister fluid with a titer similar or less than that of serum. In 25 patients with BP, the most common disease in this study, 23 cases (92%) had positive IIF on serum, 23 cases (92%) on blister fluid, and 24 cases (96%) on either serum or blister fluid. Immunoreactant titers in BP blister fluid and serum did not show significant differences (P > 0.05). Epidermal binding of immunoreactants was the most prevalent staining pattern of IIF on salt-split skin (92%) in BP. CONCLUSIONS: From the findings of this study, the blister fluid of patients with SABD can be used for IIF. Although IIF sensitivity on blister fluid is no more than that on serum, the performance of this test on blister fluid in addition to serum may reduce the number of false negative results of IIF found using either of these two substrates alone.
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Authors | Maryam Daneshpazhooh, Majid Shahdi, Mahnaz Aghaeepoor, Gholamreza Hasiri, Cheida Chams |
Journal | International journal of dermatology
(Int J Dermatol)
Vol. 43
Issue 5
Pg. 348-51
(May 2004)
ISSN: 0011-9059 [Print] England |
PMID | 15117364
(Publication Type: Comparative Study, Evaluation Study, Journal Article)
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Chemical References |
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Topics |
- Antibodies
(blood, immunology)
- Exudates and Transudates
(immunology)
- Female
- Fluorescent Antibody Technique, Indirect
(methods)
- Humans
- Male
- Predictive Value of Tests
- Skin Diseases, Vesiculobullous
(diagnosis)
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