Abstract |
The present study evaluates the biological responses to 10% blood volume infusion ("top-loading," n = 7) or 50% blood volume exchange transfusion (n = 8) with a new preparation of liposome-encapsulated hemoglobin (LEH) in the conscious rat. Top-loading did not affect systemic hemodynamic variables but induced transient thrombocytopenia (-36% +/- 7%; P less than 0.05) and elevation of plasma thromboxane B2 (12.4 +/- 3.8 pg/100 microliters vs. undetectable levels at the control; P less than 0.05). Rats exchanged with LEH maintained mean arterial pressure (MAP) during the 3 hr observation period, whereas control ( 0.9% NaCl or lactated Ringer's solution) animals developed hypotension (-23 +/- 5 mm Hg, P less than 0.05). The maintenance of MAP in the LEH group resulted from the opposing effects of LEH-induced increase in total peripheral resistance index (TPRI; +0.06 +/- 0.01 mm Hg/ml/min/kg; P less than 0.05), and decreased cardiac index, (Cl, -25 +/- 6 ml/min/kg, P less than 0.05). In contrast, the decline in MAP in the control groups could be attributed to the lower Cl (-65 +/- 8 ml/min/kg; P less than 0.05). All rats developed metabolic acidosis (base excess of -9.7 +/- 0.6 mmol/liter; P less than 0.05), which was transient in the LEH group but progressive in the control groups. These data support the possible use of LEH as a blood substitute when massive transfusions are needed.
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Authors | R Rabinovici, A S Rudolph, F S Ligler, E F Smith 3rd, G Feuerstein |
Journal | Circulatory shock
(Circ Shock)
Vol. 37
Issue 2
Pg. 124-33
(Jun 1992)
ISSN: 0092-6213 [Print] United States |
PMID | 1511503
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Substitutes
- Hemoglobins
- Lactates
- Liposomes
- Phosphatidylcholines
- Lactic Acid
- Sodium Chloride
- Thromboxane B2
- 6-Ketoprostaglandin F1 alpha
- 1,2-distearoyllecithin
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Topics |
- 6-Ketoprostaglandin F1 alpha
(analysis)
- Animals
- Blood Pressure
(drug effects)
- Blood Substitutes
(pharmacology)
- Exchange Transfusion, Whole Blood
- Heart Rate
(drug effects)
- Hemoglobins
(administration & dosage, chemistry, pharmacology)
- Lactates
(pharmacology)
- Lactic Acid
- Liposomes
- Male
- Phosphatidylcholines
- Rats
- Rats, Inbred Strains
- Sodium Chloride
(pharmacology)
- Thromboxane B2
(analysis)
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