Evidence for a rhesus monkey model of asymptomatic tuberculosis.

Abstract	Rhesus monkeys (RM) were inoculated intrabronchially with graded doses of Mycobacterium tuberculosis (MTB) strains Erdman and H37Rv in an effort to produce a model of asymptomatic tuberculosis infection. Erdman strain produced active disease within 7-11 weeks regardless of dose. Low doses of H37Rv resulted in asymptomatic infections; high doses produced active disease within 11 weeks. Over a 4-month period of post-inoculation study, MTB culture-filtrate protein (CFP)-stimulated bronchoalveolar lavage cells (BALC) and blood mononuclear cells (PBMC) from monkeys with active disease (30 cfu Erdman-inoculated) or asymptomatic infection (200 cfu H37Rv-inoculated) produced similar significant quantities of mRNA encoding for IFN-gamma or TNF-alpha, but insignificant quantities of IL-4 mRNA. Differences were observed in antigen-induced in vitro blastogenic responses and serum anti-lipoarabinomannan (LAM) antibody responses in animals with active compared with asymptomatic MTB infections. The results indicate that RM are a good model for the study of asymptomatic tuberculosis infections using low doses of H37Rv.
Authors	Bobby J Gormus, James L Blanchard, Xavier H Alvarez, Peter J Didier
Journal	Journal of medical primatology (J Med Primatol) Vol. 33 Issue 3 Pg. 134-45 (Jun 2004) ISSN: 0047-2565 [Print] Denmark
PMID	15102070 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Cytokines DNA Primers Immunoglobulin G Immunoglobulin M Lipopolysaccharides RNA, Messenger lipoarabinomannan DNA
Topics	Animals Body Burden Bronchoalveolar Lavage Fluid (cytology) Cytokines (genetics) DNA (genetics) DNA Primers Disease Models, Animal Electrophoresis, Agar Gel Enzyme-Linked Immunosorbent Assay Immunoglobulin G (immunology) Immunoglobulin M (immunology) Immunohistochemistry Immunosuppression Therapy Leukocytes, Mononuclear (immunology) Lipopolysaccharides (immunology) Lymphocyte Activation (immunology) Macaca mulatta Microscopy, Confocal Mycobacterium tuberculosis (genetics) RNA, Messenger (genetics, metabolism) Reverse Transcriptase Polymerase Chain Reaction Time Factors Tuberculosis (immunology, pathology)

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