Abstract | PURPOSE: MATERIALS AND METHODS: The CDDP resistant cell line JKT/DDP was established from the highly metastatic human testicular seminoma cell line JKT-HM by long-term intermittent administration of low dose CDDP. Growth curves, CDDP sensitivity and intracellular CDDP concentrations were compared in JKT/DDP, the seminoma cell line JKT-1 and JKT-HM cells. Expression of the mRNA of MDR1, MRP, LRP and GST-pi was also compared. RESULTS: The growth curve and doubling time of JKT/DDP were similar to those of JKT-1 and JKT-HM in culture without CDDP but different with CDDP added to the culture. Morphologically cytoplasm segmentation and chromatin aggregation in JKT-1 and JKT-HM were observed when CDDP was present, while JKT/DDP cells were spindle-shaped and showed cobblestone growth. The IC50 for CDDP determined by the collagen gel drop embedded drug sensitivity test showed that JKT/DDP was more resistant to CDDP than the other 2 cell lines. The expression of MDR1, MRP and GST-pi was higher than in JKT-1 or JKT-HM cells and the intracellular CDDP concentration was lower in JKT/DDP. CONCLUSIONS: The JKT/DDP cell line acquired CDDP resistance by increased cytoplasmic CDDP metabolism.
|
Authors | Tatsuya Kobayashi, Tomohiro Fujii, Yoshimasa Jo, Keigo Kinugawa, Masato Fujisawa |
Journal | The Journal of urology
(J Urol)
Vol. 171
Issue 5
Pg. 1929-33
(May 2004)
ISSN: 0022-5347 [Print] United States |
PMID | 15076314
(Publication Type: Journal Article)
|
Chemical References |
- Antineoplastic Agents
- Cisplatin
|
Topics |
- Antineoplastic Agents
(therapeutic use)
- Cisplatin
(therapeutic use)
- Drug Resistance, Neoplasm
- Humans
- Male
- Seminoma
(drug therapy, pathology)
- Testicular Neoplasms
(drug therapy, pathology)
- Tumor Cells, Cultured
|