Abstract |
In this study, we have shown that a paraneoplastic cerebellar degeneration (PCD)-associated antigen, pcd17, binds to a cell cycle-related protein, MRG15. MRG15 derepresses the E2F-responsive B-myb promoter. The pcd17 antigen inhibits the derepression of the B-myb transcriptional activity by MRG15, and, as a result, pcd17 represses the promoter. Delivery of anti-Purkinje cell antibodies (anti-Yo) into the cells inhibits the repression of B-myb promoter activity by pcd17. Because derepression of the B-myb promoter has been implicated in neuronal death, the results suggest the possible role of the antibodies in the pathogenesis of PCD.
|
Authors | Koichiro Sakai, Yoko Kitagawa, Shinji Saiki, Misuzu Saiki, Genjiro Hirose |
Journal | Neurobiology of disease
(Neurobiol Dis)
Vol. 15
Issue 3
Pg. 529-33
(Apr 2004)
ISSN: 0969-9961 [Print] United States |
PMID | 15056460
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Cell Cycle Proteins
- Chromosomal Proteins, Non-Histone
- DNA-Binding Proteins
- E2F Transcription Factors
- Immunoglobulin G
- Neoplasm Proteins
- Nerve Tissue Proteins
- Trans-Activators
- Transcription Factors
|
Topics |
- Animals
- COS Cells
- Cell Cycle Proteins
- Chlorocebus aethiops
- Chromosomal Proteins, Non-Histone
(drug effects, metabolism)
- DNA-Binding Proteins
(drug effects, immunology, metabolism)
- E2F Transcription Factors
- Immunoglobulin G
(pharmacology)
- Neoplasm Proteins
(immunology)
- Nerve Degeneration
(metabolism, pathology)
- Nerve Tissue Proteins
(metabolism)
- Paraneoplastic Cerebellar Degeneration
(physiopathology)
- Promoter Regions, Genetic
- Purkinje Cells
(immunology, pathology)
- Trans-Activators
(drug effects, metabolism)
- Transcription Factors
(metabolism)
- Two-Hybrid System Techniques
|