Loss of tumorigenicity of murine colon carcinoma MC38/0 cell line after transduction with a retroviral vector carrying murine IL-12 genes.

Abstract

Cells of transplantable MC38 colon carcinoma of C57BL/6 mice were adapted to growth in vitro as the MC38/0 cell line. Along the establishing process, MC38/0 cells preserved their tumorigenicity. After transduction with a retroviral vector carrying murine interleukin 12 (mIL-12) genes and further selection, stable MC38/IL-12 transductant cells were obtained. These cells produced IL-12 (approx. 2500 ng/ml/5x10(5) cells/48 h) as evaluated in the optimized bioassay. After subcutaneous inoculation into syngeneic mice, the IL-12-modified cells demonstrated reduced tumorigenicity as compared to parental MC38/0 cells. Mice that rejected the MC38/IL-12 tumour became protected against subsequent challenge with MC38/0 cells. The obtained data indicate that the IL-12-transduced murine colon carcinoma cells could be used both as a model tumour for the study of mechanisms of anticancer immunity and/or as an adjuvant to cancer vaccines.

• Authors: E Pajtasz-Piasecka, A Szyda, J Rossowska, A Krawczenko, M Indrová, P Grabarczyk, P Wysocki, A Mackiewicz, D Duś
• Journal: Folia biologica (Folia Biol (Praha)) Vol. 50 Issue 1 Pg. 7-14 ( 2004) ISSN: 0015-5500 [Print] Czech Republic
• PMID: 15055737 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Luminescent Proteins
• Green Fluorescent Proteins
• Interleukin-12
• Interferon-gamma

Topics

• Animals
• Cell Line, Tumor
• Colonic Neoplasms (genetics, immunology, metabolism, pathology)
• Cytokines (metabolism)
• Female
• Genes, MHC Class I
• Genetic Vectors
• Green Fluorescent Proteins
• Interferon-gamma (metabolism)
• Interleukin-12 (genetics, metabolism)
• Luminescent Proteins (genetics, metabolism)
• Mice
• Mice, Inbred C57BL
• Retroviridae (genetics)
• Transduction, Genetic