Abstract | BACKGROUND:
Ischemia-reperfusion injury (IRI) can result in severe organ dys- or nonfunction. Interaction of leukocytes and endothelial cells mediated by E-selectin appears to be a key step for disturbed microcirculation. Therefore we studied gene and protein expression as well as localization of E-selectin during intestinal IRI. METHODS: Intestinal tissue samples were obtained from extracorporeal perfused intestines (cold ischemia time [ CIT] 2 or 20 hours, each n = 5) and additionally in intestinal transplanted pigs ( CIT 2 or 20 hours, each n = 1). Mucosal damage was graded according to the Chiu classification. E-selectin mRNA was determined by PCR and quantitative RT-PCR. Localization of E-selectin mRNA was performed by in situ hybridization and of the protein by immunohistochemistry. RESULTS: Histologically, mucosal damage occurred during reperfusion and was earlier and more severe after 20 hours of CIT. E-selectin mRNA expression was detected by PCR already after laparotomy and was elevated after reperfusion. Interestingly, mRNA expression was already increased after 20 hours of CIT. E-selectin mRNA was localized to the luminal surface of muscular, submucosal, and mucosal endothelial cells and the protein was detected on submucosal arterial endothelium as early as 2 hours after reperfusion. CONCLUSION: Prolongation of CIT results in more severe mucosal damage during reperfusion, which is associated with protein expression of E-selection that might be used as a marker for activated endothelial cells. Increased E-selectin mRNA at end of 20 hours of CIT might indicate a preactivated state of endothelial cells potentially triggered by bacterial translocation or products.
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Authors | F Braun, M Hosseini, E Wieland, B Sattler, S Laabs, T Lorf, A R Müller, F Fändrich, B Kremer, B Ringe |
Journal | Transplantation proceedings
(Transplant Proc)
Vol. 36
Issue 2
Pg. 265-6
(Mar 2004)
ISSN: 0041-1345 [Print] United States |
PMID | 15050129
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- E-Selectin
- RNA, Messenger
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Topics |
- Animals
- E-Selectin
(genetics)
- Intestinal Mucosa
(blood supply, pathology)
- Intestines
(blood supply, physiology, transplantation)
- Ischemia
- RNA, Messenger
(genetics)
- Reperfusion Injury
(genetics)
- Swine
- Transcription, Genetic
(genetics)
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