Elevated plasma levels of
homocysteine are a risk factor for
cardiovascular diseases,
neural tube defects, and
Alzheimer's disease. The transsulfuration pathway converts
homocysteine to
cysteine, and approximately 50% of the
cysteine in
glutathione is derived from
homocysteine in human liver cells, which suggests the hypothesis that defects in the transsulfuration pathway perturb redox homeostasis. To test this hypothesis, we examined a murine model for
hyperhomocysteinemia in which the gene encoding the first
enzyme in the transsulfuration pathway,
cystathionine beta-synthase (CBS), has been disrupted. Limited metabolite profiling and CBS expression studies in liver, kidney, and brain reveal tissue-specific differences in the response to Cbs disruption. Homozygous disruption of Cbs lowered
cysteine concentration in all three organs.
Glutathione concentration was diminished in liver and brain, thus affecting the redox buffering capacity in these organs, whereas the approximately twofold higher
glutathione synthesis capacity in kidney helped preserve the
glutathione pool size despite loss of the transsulfuration pathway in this organ. In contrast, disruption of a single Cbs allele elicited only minor redox perturbations. Furthermore, the Cbs+/- genotype did not confer a significant disadvantage compared with the Cbs+/+ genotype in hepatocytes challenged by oxidative stress from exposure to
tertiary butylhydroperoxide. These studies provide evidence that homozygous disruption of Cbs perturbs redox homeostasis and reduces
cysteine levels, raising the possibility that these changes may be important in the etiology of the clinical manifestations of
CBS deficiency.