HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effect of C-reactive protein on chemokine expression in human aortic endothelial cells.

Abstract
Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRP. Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31%, P<0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity.
AuthorsSridevi Devaraj, Pappanaicken R Kumaresan, Ishwarlal Jialal
JournalJournal of molecular and cellular cardiology (J Mol Cell Cardiol) Vol. 36 Issue 3 Pg. 405-10 (Mar 2004) ISSN: 0022-2828 [Print] England
PMID15010279 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • CCL2 protein, human
  • Chemokine CCL2
  • Interleukin-8
  • NF-kappa B
  • Peptides
  • RNA, Messenger
  • SN50 peptide
  • Sesquiterpenes
  • parthenolide
  • C-Reactive Protein
Topics
  • Aorta (cytology)
  • Arteriosclerosis (metabolism)
  • C-Reactive Protein (pharmacology)
  • Cell Adhesion (drug effects)
  • Cells, Cultured
  • Chemokine CCL2 (biosynthesis, genetics)
  • Endothelial Cells (metabolism)
  • Gene Expression Regulation (drug effects)
  • Humans
  • Interleukin-8 (biosynthesis, genetics)
  • NF-kappa B (genetics, metabolism)
  • Peptides (pharmacology)
  • RNA, Messenger (biosynthesis, genetics)
  • Sesquiterpenes (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: