[Effect of EGF on ubiquitination and proteasome-dependent degradation of phospholipase C gamma1 in A431 cells].

Abstract	Phospholipase C gamma 1 (PLC gamma 1), an enzyme participating in phosphoinositide turnover, is one of the key elements in cell signaling. Here it is shown that treatment of A431 carcinoma cells with proteasome inhibitors Mg132 and lactacystin results in increasing the PLC gamma 1 intracellular level. Simultaneously, several additional bands with lower electrophoretic mobilities were detected on immunoblots, using anti-PLC gamma 1 antibodies. PLC gamma 1 ubiquitinilation was shown using immunoprecepitation. In control A 431 cells, PLC gamma 1 is ubiquitinilated, but the addition of EGF greatly induces the ubiquitinilation of the protein. Association of PLC gamma 1 with ubiquitin-ligase c-Cb1 was shown. Dynamics of ubiquitinilation under EGF treatment is in a close agreement with that of association of PLC gamma 1 and c-Cb1. It is concluded that PLC gamma 1 is ubiquitinilated and degraded by proteasomes. PLC gamma 1 ubiquitinilation is an EGF-dependent process.
Authors	A L Evdonin, N V Tsupkina, N N Nikol'skiĭ, N D Medvedeva
Journal	Tsitologiia (Tsitologiia) Vol. 45 Issue 10 Pg. 1013-8 ( 2003) ISSN: 0041-3771 [Print] Russia (Federation)
Vernacular Title	Vliianye EFR na ubikvitinilirovanie i proteasomozavisimuiu degradatsiiu fosfolipazy C gamma1 v kletkakh A431.
PMID	14989173 (Publication Type: Journal Article)
Chemical References	Cysteine Proteinase Inhibitors Leupeptins Multienzyme Complexes Ubiquitins lactacystin Epidermal Growth Factor Type C Phospholipases Phospholipase C gamma Cysteine Endopeptidases Proteasome Endopeptidase Complex benzyloxycarbonylleucyl-leucyl-leucine aldehyde Acetylcysteine
Topics	Acetylcysteine (analogs & derivatives, pharmacology) Carcinoma, Squamous Cell (metabolism) Cell Line, Tumor Cysteine Endopeptidases (metabolism) Cysteine Proteinase Inhibitors (pharmacology) Epidermal Growth Factor (metabolism) Epithelial Cells (drug effects, metabolism) Humans Leupeptins (pharmacology) Multienzyme Complexes (metabolism) Phospholipase C gamma Proteasome Endopeptidase Complex Type C Phospholipases (metabolism) Ubiquitins (metabolism)

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