Abstract | PURPOSE: METHODS: Intimal hyperplasia in the abdominal aortae of swine was created by a combination of fat-supplemented diet and balloon catheter injury prior to PDT. Swine were randomly allocated into one of three groups which received either: (i) both drug and light ( PDT), (ii) drug only, or (iii) light only. Twenty-four hours following administration of the photosensitizer PHOTOFRIN ( porfimer sodium) at 2.5 mg/kg, two distinct 1 cm spots on the posterior wall of the abdominal aorta were illuminated by an argon pumped dye laser tuned to 630 nm for an energy fluence of 120 J/cm2. After three or six months, swine were sacrificed, perfusion fixed, and had their aortae removed for light and electron microscopy. RESULTS: Intimal hyperplasia reduction following PDT persisted for the three or six months follow up period. Experimental vessels receiving PDT showed a 26.0+/-4.5% ( n = 2, ie. four spots) and 30.8+/-5.4% ( n = 1, ie. two spots) smaller percent intimal area after three or six months of recovery, respectively. Control groups receiving either light or drug only showed less than a 6% difference in percent intimal area. Medial and adventitial layers were unaffected in all groups. Electron microscopy demonstrated that the endothelium or endothelial-like cells had regenerated in both the posterior and adjacent areas of the abdominal aortae with no clear difference between them. CONCLUSIONS: These findings suggest that PDT may be beneficial in reducing intimal hyperplasia for up to three or six months in swine.
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Authors | Justin Cheung, Mary Todd, Robert Turnbull, Beth Allison, Lorna Mornin, Anna-Maria Carvalho, George Spurr, York Hsiang |
Journal | Journal of photochemistry and photobiology. B, Biology
(J Photochem Photobiol B)
Vol. 73
Issue 3
Pg. 141-7
(Feb 20 2004)
ISSN: 1011-1344 [Print] Switzerland |
PMID | 14975402
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Validation Study)
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Chemical References |
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Topics |
- Animals
- Aorta, Abdominal
(drug effects, pathology, radiation effects)
- Dihematoporphyrin Ether
(therapeutic use)
- Feasibility Studies
- Hematoporphyrin Photoradiation
(methods)
- Hyperplasia
(drug therapy, pathology)
- Photochemotherapy
(methods)
- Pilot Projects
- Swine
- Treatment Outcome
- Tunica Intima
(drug effects, pathology, radiation effects)
- Vascular Diseases
(drug therapy, pathology)
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