TP53 mutations in early-stage ovarian carcinoma, relation to long-term survival.

Abstract

We conducted the present study to evaluate the frequency and prognostic importance on long-term survival of TP53 mutations and TP53 protein accumulation in a cohort of 178 patients with early-stage ovarian carcinomas. TP53 mutations scored as aberrant temporal temperature gradient gel electrophoresis pattern from all exons were observed in 39.9% of the tumours. Full screening of exons 5-8, followed by sequencing, was successful in 135 cases, and 48 mutations altering the protein were detected in 39 cases (28.9%). TP53 mutations were slightly less common in the Federation of Gynecologists and Obstetricians stage IA than in IB/IC (P=0.05). No significant correlations with histological type, grade of differentiation, DNA ploidy status or age at diagnosis were found. TP53 protein accumulation analysed by immunohistochemistry was found in 32.6% of all tumours, and was a poor predictor of TP53 mutations with 56.4% sensitivity, 77.1% specificity, 50% positive predictive value and 81.3% negative predictive value. Neither TP53 mutations nor TP53 protein accumulation influenced the prognosis significantly in this group of patients.

• Authors: Y Wang, A Helland, R Holm, H Skomedal, V M Abeler, H E Danielsen, C G Tropé, A-L Børresen-Dale, G B Kristensen
• Journal: British journal of cancer (Br J Cancer) Vol. 90 Issue 3 Pg. 678-85 (Feb 09 2004) ISSN: 0007-0920 [Print] England
• PMID: 14760384 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Tumor Suppressor Protein p53

Topics

• Aged
• Cohort Studies
• DNA Mutational Analysis
• DNA, Neoplasm
• Electrophoresis, Gel, Two-Dimensional
• Exons
• Female
• Genes, p53 (genetics)
• Humans
• Immunohistochemistry
• Middle Aged
• Neoplasm Staging
• Ovarian Neoplasms (genetics, pathology)
• Ploidies
• Predictive Value of Tests
• Prognosis
• Survival Analysis
• Tumor Suppressor Protein p53 (analysis, genetics)