Geminivirus DNA replication and cell cycle interactions.

Abstract

The Geminiviridae family includes a large number of viruses that infect plants and have a unique geminate virion particle, a single-stranded genome of approximately 2.6-3.0 kb, and replicate through a rolling-circle mechanism. Since they encode for just a few proteins (4-6 depending on the members that belong to four different genera), a rich variety of interactions has evolved between viral proteins and host factors to develop the virus replicative cycle. Among them, we have been particularly interested so far: (i). in the interference with cell cycle regulatory proteins of the retinoblastoma-related (RBR)/E2F pathway and (ii). in the interaction with host DNA replication factors necessary for the assembly of a functional replication complex at the viral origin of DNA replication during the rolling-circle stage. Yeast two-hybrid assays revealed that wheat dwarf virus RepA protein, but nor Rep protein, interacts with plant RBR protein. Interestingly, deletion of the C-terminal domain of Rep confers the truncated protein the ability to interact with RBR, suggesting that this domain may hinder the LXCXE RBR-binding motif. Secondary structure predictions support such a possibility.

• Authors: Crisanto Gutierrez, Elena Ramirez-Parra, M Mar Castellano, Andres P Sanz-Burgos, Alejandro Luque, Riccardo Missich
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 98 Issue 2 Pg. 111-9 (Feb 04 2004) ISSN: 0378-1135 [Print] Netherlands
• PMID: 14741123 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Cycle Proteins
• Retinoblastoma Protein

Topics

• Amino Acid Sequence
• Cell Cycle (physiology)
• Cell Cycle Proteins (metabolism)
• DNA Replication (physiology)
• Geminiviridae (genetics, growth & development, metabolism, physiology)
• Genome, Viral
• Molecular Sequence Data
• Replication Origin
• Retinoblastoma Protein (metabolism)
• Two-Hybrid System Techniques
• Virus Replication (genetics)