Abstract |
Zwitterionic polysaccharides (Zps) from pathogenic bacteria, such as Bacteroides fragilis, are virulence factors responsible for abscess formation associated with intra-abdominal sepsis. The underlying cellular mechanism for abscess formation requires T cell activation. Conversely, abscess formation can be prevented by prophylactic s.c. injection of purified Zps alone, a process also dependent on T cells. Hence, the modulatory role of T cells in abscess formation was investigated. We show that Zps interact directly with T cells with fast association/dissociation kinetics. Vbeta repertoire analysis using RT-PCR demonstrates that Zps have broad Vbeta usage. Zps-specific hybridomas responded to a variety of other Zps, but not to a nonzwitterionic polysaccharide, indicating cross-reactivity between different Zps. Furthermore, Zps-reactive T cell hybridomas could effectively transfer protection against abscess formation. Analysis of the proliferative capacity of T cells recovered from Zps-treated animals revealed that these T cells are anergic to subsequent stimulation by the different Zps or to alloantigens in an MLR. This anergic response was relieved by addition of IL-2. Taken together, the data show that this class of polysaccharides interacts directly with T cells in a nonbiased manner to elicit an IL-2-dependent anergic response that confers protection against abscess formation.
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Authors | Francesca Stingele, Blaise Corthésy, Nicole Kusy, Steven A Porcelli, Dennis L Kasper, Arthur O Tzianabos |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 172
Issue 3
Pg. 1483-90
(Feb 01 2004)
ISSN: 0022-1767 [Print] United States |
PMID | 14734725
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Bacterial
- Ions
- Peptides
- Polysaccharides, Bacterial
- Receptors, Antigen, T-Cell, alpha-beta
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Topics |
- Abscess
(immunology, prevention & control)
- Animals
- Antigens, Bacterial
(immunology)
- Bacteroides Infections
(immunology, microbiology, prevention & control)
- Bacteroides fragilis
(immunology)
- Cell Communication
(immunology)
- Cell Line, Tumor
- Clonal Anergy
(immunology)
- Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
(immunology)
- Hybridomas
- Ions
- Lymphocyte Activation
(immunology)
- Male
- Mice
- Peptides
(pharmacology)
- Pneumococcal Infections
(immunology, prevention & control)
- Polysaccharides, Bacterial
(immunology, pharmacology)
- Rats
- Rats, Inbred Lew
(microbiology)
- Rats, Wistar
- Receptors, Antigen, T-Cell, alpha-beta
(biosynthesis)
- T-Lymphocytes
(immunology, metabolism, microbiology)
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