DNA polymerase eta is involved in hypermutation occurring during immunoglobulin class switch recombination.
|
| Abstract |
Base substitutions, deletions, and duplications are observed at the immunoglobulin locus in DNA sequences involved in class switch recombination (CSR). These mutations are dependent upon activation-induced cytidine deaminase (AID) and present all the characteristics of the ones observed during V gene somatic hypermutation, implying that they could be generated by the same mutational complex. It has been proposed, based on the V gene mutation pattern of patients with the cancer-prone xeroderma pigmentosum variant (XP-V) syndrome who are deficient in DNA polymerase eta (pol eta), that this enzyme could be responsible for a large part of the mutations occurring on A/T bases. Here we show, by analyzing switched memory B cells from two XP-V patients, that pol eta is also an A/T mutator during CSR, in both the switch region of tandem repeats as well as upstream of it, thus suggesting that the same error-prone translesional polymerases are involved, together with AID, in both processes.
|
|---|---|
| Authors | Ahmad Faili, Said Aoufouchi, Sandra Weller, Françoise Vuillier, Anne Stary, Alain Sarasin, Claude-Agnès Reynaud, Jean-Claude Weill |
| Journal | The Journal of experimental medicine (J Exp Med) Vol. 199 Issue 2 Pg. 265-70 (Jan 19 2004) ISSN: 0022-1007 [Print] United States |
| PMID | 14734526 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!