Abstract | BACKGROUND: METHODS:
Acute pancreatitis was induced in male Wistar rats by intraductal administration of 5% sodium taurocholate. In another experimental group 3000 U/Kg alpha-amylase was i.v. administered. The concentrations of XDH, XOD and alpha-amylase in plasma and AF and myeloperoxidase (MPO) in lung have been evaluated. In additional experiments, the effect of peritoneal lavage and the absorption of alpha-amylase present in the AF by an isolated intestine have been determined. RESULTS: CONCLUSIONS: During the early stages of acute pancreatitis, alpha-amylase absorbed from AF through the gastrointestinal tract could interfere with the binding of XDH/XOD attached to glycoproteins of the endothelial cells. Proteolytic enzymes convert XDH into its oxidase form promoting an increase in circulating XOD that has been reported to be one of the mechanisms involved in the triggering of the systemic inflammatory process.
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Authors | Susana Granell, Oriol Bulbena, Meritxell Genesca, Luis Sabater, Juan Sastre, Emilio Gelpi, Daniel Closa |
Journal | BMC gastroenterology
(BMC Gastroenterol)
Vol. 4
Pg. 1
(Jan 19 2004)
ISSN: 1471-230X [Electronic] England |
PMID | 14728722
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Taurocholic Acid
- Peroxidase
- Xanthine Dehydrogenase
- Xanthine Oxidase
- Amylases
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Topics |
- Acute Disease
- Amylases
(pharmacokinetics)
- Animals
- Ascitic Fluid
(metabolism)
- Binding, Competitive
- Endothelium
(enzymology)
- Intestinal Absorption
- Lung
(enzymology)
- Male
- Pancreatitis
(chemically induced, enzymology)
- Peroxidase
(metabolism)
- Rats
- Rats, Wistar
- Taurocholic Acid
- Xanthine Dehydrogenase
(metabolism)
- Xanthine Oxidase
(metabolism)
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