Abstract | UNLABELLED: MATERIAL AND METHODS: Male Wistar rats (weighting 280 to 300 g, n = 40) were used. Each rat was mounted on a stereotaxic frame under general anesthesia. DAVF model (Spetzler et al.) was made by common carotid artery-external jugular vein anastomosis, bipolar coagulation of the vein draining the transverse sinus, and superior sagittal sinus thrombosis (SSS). SSS was thrombosed by a hemostatic agent through 16-gauge needle. Venous hypertension was induced in 30 rats, which were divided into two experimental groups; (1) immunohistological study group (n = 15) and (2) angiography group (n = 15). Immunohistological analysis was performed by VEGF antibody 1 week after, and angiography was done 90 days after the surgery. Developing of angiographical DAVF was observed with the magnifying X-ray camera. Each 5 rats served as sham-operated controls, which received a similar surgery without induction of venous hypertension. RESULTS:
VEGF expression and DAVF were not observed in sham group. In immunohistological study group, VEGF expression in the endothelium and the connective tissues of the dura mater in the five rats (33%) and in the neurons in the eleven rats (73%) of the cerebral cortex and the basal ganglia were identified. In angiography group, DAVF formed in 6 among 15 rats (40%). CONCLUSION: The findings of this study provide the first experimental evidence that angiogenic growth factors VEGF may participate in the genesis of DAVF. These results suggest a novel strategy for the management and prevention of DAVF and related disorders.
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Authors | Yasushi Shin, Ryunosuke Uranishi, Hiroyuki Nakase, Toshisuke Sakaki |
Journal | No to shinkei = Brain and nerve
(No To Shinkei)
Vol. 55
Issue 11
Pg. 946-52
(Nov 2003)
ISSN: 0006-8969 [Print] Japan |
PMID | 14727534
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Vascular Endothelial Growth Factors
|
Topics |
- Animals
- Basal Ganglia
(metabolism)
- Central Nervous System Vascular Malformations
(etiology, metabolism)
- Disease Models, Animal
- Dura Mater
(metabolism)
- Male
- Rats
- Rats, Wistar
- Vascular Endothelial Growth Factors
(biosynthesis)
- Venous Pressure
(physiology)
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