Technetium-99m sestamibi single photon emission computed tomography findings correlated with P-glycoprotein expression, encoded by the multidrug resistance gene-1 messenger ribonucleic acid, in intracranial meningiomas.

Abstract

The present study evaluated whether technetium-99m sestamibi (99mTc-MIBI) single photon emission computed tomography (SPECT) characteristics of intracranial meningioma are correlated with the histological malignancy, proliferative potential, and P-glycoprotein (Pgp) expression, encoded by the multidrug resistance gene-1 (MDR-1) messenger ribonucleic acid (mRNA). Twenty-one patients with intracranial meningiomas, including 17 benign and four nonbenign meningiomas, underwent 99mTc-MIBI SPECT imaging at 15 minutes (early) and 3 hours (delayed) after injection. The tumor-to-normal pituitary gland ratio was calculated on both early (ER) and delayed (DR) images. Retention index (RI) was calculated using the following formula: (DR - ER)/ER x 100%. Meningioma specimens were examined by immunohistochemistry using anti-Pgp and MIB-1 monoclonal antibody. MDR-1 mRNA expression was also investigated using reverse transcription-polymerase chain reaction assay. 99mTc-MIBI was highly accumulated and retained in the tumors. 99mTc-MIBI SPECT findings were not related to MIB-1 labeling index. 99mTc-MIBI SPECT RI of the Pgp-positive group (-9.12 +/- 22.27%) was significantly lower than that of the Pgp-negative group (28.79 +/- 22.80%) (p = 0.0016). No significant difference was seen in ER and DR between the positive and negative groups. These results show that 99mTc-MIBI may not be useful for determining proliferative potential and histological malignancy, but could predict anticancer drug resistance related to the expression of MDR-1 mRNA and its gene product Pgp in patients with intracranial meningiomas.

• Authors: Katsuzo Kunishio, Kuniaki Morisaki, Yoshihito Matsumoto, Seigo Nagao, Yoshihiro Nishiyama
• Journal: Neurologia medico-chirurgica (Neurol Med Chir (Tokyo)) Vol. 43 Issue 12 Pg. 573-80; discussion 581 (Dec 2003) ISSN: 0470-8105 [Print] Japan
• PMID: 14723263 (Publication Type: Journal Article)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• DNA Primers
• RNA, Messenger
• Radiopharmaceuticals
• Technetium Tc 99m Sestamibi

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (genetics)
• Adult
• Aged
• Brain Neoplasms
• DNA Primers (genetics)
• Female
• Genes, MDR (genetics)
• Humans
• Immunohistochemistry
• Male
• Meningeal Neoplasms (diagnosis, genetics, metabolism)
• Meningioma (diagnosis, genetics, metabolism)
• Middle Aged
• RNA, Messenger (genetics)
• Radiopharmaceuticals (pharmacokinetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Technetium Tc 99m Sestamibi (pharmacokinetics)
• Tomography, Emission-Computed, Single-Photon