HLA-DQ beta 57 in Hispanic patients with insulin-dependent diabetes mellitus.

Abstract

OBJECTIVE:

The purpose of our study was to investigate the distribution of HLA-DQ beta-chain amino acid residue 57 (HLA-DQ beta 57) as a genetic marker of susceptibility for insulin-dependent diabetes mellitus in the Hispanic population.

STUDY DESIGN:

Fifteen patients of Puerto Rican descent with juvenile-onset insulin-dependent diabetes mellitus underwent human leukocyte antigen typing for HLA-DQ beta 57 by polymerase chain reaction amplification of the target genomic DQ sequence followed by hybridization of the polymerase chain reaction product to phosphorus 32-labeled allele-specific oligonucleotide probes. A control group of 44 Hispanic adults without diabetes who were undergoing human leukocyte antigen typing for tissue donation were concurrently typed for comparison.

RESULTS:

The Hispanic insulin-dependent diabetes mellitus group showed a significant increase in homozygosity for a non-aspartate amino acid (p = 0.023) over a control group of Hispanic subjects without diabetes. A high rate of heterozygosity for aspartate (53.3%) is found in Hispanic subjects with insulin-dependent diabetes mellitus as well.

CONCLUSIONS:

HLA-DQ beta 57 in the Hispanic population has a distribution distinct from HLA-DQ beta 57 in the Caucasian population. A single aspartate is not protective against insulin-dependent diabetes mellitus in Hispanic subjects.

• Authors: G Lee, F N Shamma, M P Diamond, J T Lee
• Journal: American journal of obstetrics and gynecology (Am J Obstet Gynecol) Vol. 167 Issue 6 Pg. 1565-70 (Dec 1992) ISSN: 0002-9378 [Print] United States
• PMID: 1471667 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Genetic Markers
• HLA-DQ Antigens
• HLA-DQ beta-Chains
• HLA-DQbeta antigen
• Molecular Probes

Topics

• Amino Acid Sequence
• Amino Acids (genetics)
• Base Sequence
• Diabetes Mellitus, Type 1 (ethnology, genetics, immunology)
• Genetic Markers
• Genetic Predisposition to Disease
• HLA-DQ Antigens (analysis, genetics)
• HLA-DQ beta-Chains
• Heterozygote
• Hispanic or Latino
• Homozygote
• Humans
• Molecular Probes (genetics)
• Molecular Sequence Data