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[Detecting HPV DNA in tissues of external genital squamous cell carcinoma in situ by PCR-RFLP technique].

AbstractOBJECTIVE:
To study the relationship between the infection of human papillomavirus (HPV) and external genital squamous cell carcinoma in situ.
METHODS:
HPV DNA was detected with the consensus primer by polymerase chain reaction (PCR) and its type was determined by restriction fragment length polymorphism (RFLP).
RESULTS:
HPV DNA was detected and HPV16 was the most frequently identified type in 29 (56.9%) of 51 cases of external genital squamous cell carcinoma in situ. HPV DNA was positive in 22 (55%) of 40 cases of bowenoid papulosis, among which 20 were of HPV16 type, one of HPV31 type, and one of HPV6 + 16 type. HPV DNA was positive in all 5 cases (100%) of Bowen's disease, among which 4 were HPV16 type and one of HPV6 + 16 type. HPV DNA was positive in 2 (33.3%) of 6 Queyrat erythroplasia cases and all were of HPV16 type.
CONCLUSION:
HPV16 infection is strongly associated with the external genital squamous cell carcinoma in situ including bowenoid papulosis, Bowen's disease, and Queyrat erythroplasia.
AuthorsJia-bi Wang, Xiao-hong Man, Yue-hua Liu, Kai Fang
JournalZhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 25 Issue 6 Pg. 667-70 (Dec 2003) ISSN: 1000-503X [Print] China
PMID14714308 (Publication Type: English Abstract, Journal Article)
Chemical References
  • DNA Probes, HPV
  • DNA, Viral
Topics
  • Adult
  • Aged
  • Bowen's Disease (virology)
  • Carcinoma in Situ (virology)
  • Carcinoma, Squamous Cell (virology)
  • DNA Probes, HPV
  • DNA, Viral (analysis)
  • Erythroplasia (virology)
  • Female
  • Genital Neoplasms, Female (virology)
  • Genital Neoplasms, Male (virology)
  • Humans
  • Male
  • Middle Aged
  • Papillomaviridae (isolation & purification)
  • Papillomavirus Infections (virology)
  • Penile Neoplasms (virology)
  • Polymorphism, Restriction Fragment Length
  • Tumor Virus Infections (virology)
  • Vulvar Neoplasms (virology)

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