Inhibitory effect of topiramate on Lewis lung carcinoma metastasis and its relation with AQP1 water channel.

Abstract

AIM:

To study the effect of topiramate on tumor metastasis and its relation with aquaporin 1 (AQP1) water channel.

METHODS:

Lewis lung carcinoma metastatic model was used to determine the effect of topiramate on tumor growth and metastasis. Colorimetric estimation was used to investigate the action of topiramate on carbonic anhydrase (CA) activity. Western blotting and immunohistochemical analysis were used to study the influence of topiramate on AQP1 water channel expression in lungs or tumor tissues of mice bearing Lewis lung carcinoma.

RESULTS:

Treatment with topiramate (120 mg/kg/d, ig for 20 d) reduced the growth of primary tumor significantly (P<0.05). Its inhibitory rate of metastasis was 81.25 %. Topiramate inhibited CA activity in lungs of mice in a dose-dependent manner. Topiramate apparently decreased AQP1 protein expression and immunostaining in lungs or in tumor microvessel endothelial cells of mice.

CONCLUSION:

Suppression of AQP1 water channel expression may be an important pathway for the inhibitory effect of topiramate on tumor metastasis.

• Authors: Bing Ma, Yang Xiang, Tao Li, He-Ming Yu, Xue-Jun Li
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 25 Issue 1 Pg. 54-60 (Jan 2004) ISSN: 1671-4083 [Print] United States
• PMID: 14704123 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aqp1 protein, mouse
• Aquaporins
• Topiramate
• Aquaporin 1
• Fructose
• Carbonic Anhydrases

Topics

• Animals
• Aquaporin 1
• Aquaporins (metabolism)
• Carbonic Anhydrases (metabolism)
• Carcinoma, Lewis Lung (enzymology, metabolism, pathology)
• Female
• Fructose (analogs & derivatives, pharmacology)
• Lung (enzymology, metabolism)
• Mice
• Mice, Inbred C57BL
• Neoplasm Metastasis
• Topiramate