Effects of dl-praeruptorin A on nucleus factor-kappaB and tumor necrosis factor-alpha expression in ischemia-reperfusion hearts.

Abstract

AIM:

To study the effects of dl-praeruptorin A (Pd-Ia) on nucleus factor-kappaB (NF-kappaB) activativity and tumor necrosis factor-alpha (TNF-alpha) expression in ischemia-reperfusion (I/R) myocardium.

METHODS:

Langendorff's isolated rat heart was subjected to a 10-min ischemia followed by a 30-min reperfusion. NF-kappaB activity in nucleus was analyzed by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). TNF-alpha level in cytoplasm was measured by radioimmunoassay. Infiltration of neutrophils was observed using Hematoxylin-Eosin staining under optical microscope.

RESULTS:

Pd-Ia 1.0 micromol/L with 30-min preventive perfusion decreased NF-kappaB activity from 0.98+/-0.13 to 0.65+/-0.17 (P<0.05 vs solvent) and down-regulated TNF-alpha expression from 13.7+/-6.1 microg/L to 9.4+/-2.7 microg/L (P<0.01 vs solvent) under conditions with increase of coronary flow, negative inotropic action, inhibition of creatine kinase and without chronotropic action, whereas, infiltration of neutrophils was mild.

CONCLUSION:

Pd-Ia inhibited NF-kappaB activativity in I/R myocardium and led to down-regulation of TNF-alpha expression, which might be one of molecular mechanisms of Pd-Ia in cardioprotection.

• Authors: Chi Wang, Tian-Hui Chang, Xin-Hua Zhang, Huai-Liang Wang
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 25 Issue 1 Pg. 35-40 (Jan 2004) ISSN: 1671-4083 [Print] United States
• PMID: 14704120 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• Coumarins
• Drugs, Chinese Herbal
• NF-kappa B
• Tumor Necrosis Factor-alpha
• praeruptorin A
• Creatine Kinase

Topics

• Animals
• Cardiotonic Agents (pharmacology)
• Coronary Circulation (drug effects)
• Coumarins (pharmacology)
• Creatine Kinase (metabolism)
• Drugs, Chinese Herbal (pharmacology)
• Female
• Male
• Myocardial Contraction (drug effects)
• Myocardial Reperfusion Injury (etiology, metabolism, pathology)
• Myocardium (metabolism, pathology)
• NF-kappa B (biosynthesis)
• Rats
• Rats, Wistar
• Tumor Necrosis Factor-alpha (metabolism)