Abstract | AIM: METHODS: Langendorff's isolated rat heart was subjected to a 10-min ischemia followed by a 30-min reperfusion. NF-kappaB activity in nucleus was analyzed by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). TNF-alpha level in cytoplasm was measured by radioimmunoassay. Infiltration of neutrophils was observed using Hematoxylin- Eosin staining under optical microscope. RESULTS:
Pd-Ia 1.0 micromol/L with 30-min preventive perfusion decreased NF-kappaB activity from 0.98+/-0.13 to 0.65+/-0.17 (P<0.05 vs solvent) and down-regulated TNF-alpha expression from 13.7+/-6.1 microg/L to 9.4+/-2.7 microg/L (P<0.01 vs solvent) under conditions with increase of coronary flow, negative inotropic action, inhibition of creatine kinase and without chronotropic action, whereas, infiltration of neutrophils was mild. CONCLUSION:
Pd-Ia inhibited NF-kappaB activativity in I/R myocardium and led to down-regulation of TNF-alpha expression, which might be one of molecular mechanisms of Pd-Ia in cardioprotection.
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Authors | Chi Wang, Tian-Hui Chang, Xin-Hua Zhang, Huai-Liang Wang |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 25
Issue 1
Pg. 35-40
(Jan 2004)
ISSN: 1671-4083 [Print] United States |
PMID | 14704120
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cardiotonic Agents
- Coumarins
- Drugs, Chinese Herbal
- NF-kappa B
- Tumor Necrosis Factor-alpha
- praeruptorin A
- Creatine Kinase
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Topics |
- Animals
- Cardiotonic Agents
(pharmacology)
- Coronary Circulation
(drug effects)
- Coumarins
(pharmacology)
- Creatine Kinase
(metabolism)
- Drugs, Chinese Herbal
(pharmacology)
- Female
- Male
- Myocardial Contraction
(drug effects)
- Myocardial Reperfusion Injury
(etiology, metabolism, pathology)
- Myocardium
(metabolism, pathology)
- NF-kappa B
(biosynthesis)
- Rats
- Rats, Wistar
- Tumor Necrosis Factor-alpha
(metabolism)
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