Abstract |
The concentration and size distribution of infectious aerosols produced by patients with pulmonary tuberculosis (TB) has never been directly measured. We aimed to assess the feasibility of a method that we developed to collect and quantify culturable cough-generated aerosols of Mycobacterium tuberculosis. Subjects were recruited from a referral hospital and most had multidrug-resistant TB. They coughed into a chamber containing microbial air samplers while cough frequency was measured during two 5-minute sessions. Cough-generated aerosol cultures were positive in 4 of 16 subjects (25%) with smear-positive pulmonary TB. There was a rapid decrease in the cough-generated aerosol cultures within the first 3 weeks of effective treatment. Culture-positive cough aerosols were associated with lack of treatment during the previous week (p = 0.007), and there was a trend in the association with cough frequency (p = 0.08). The size distributions of these aerosols were variable, but most particle sizes were in the respirable range. Quantification of viable cough-generated aerosols is feasible and offers a new approach to study infectiousness and transmission of M. tuberculosis and other airborne pathogens.
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Authors | Kevin P Fennelly, John W Martyny, Kayte E Fulton, Ian M Orme, Donald M Cave, Leonid B Heifets |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 169
Issue 5
Pg. 604-9
(Mar 01 2004)
ISSN: 1073-449X [Print] United States |
PMID | 14656754
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Aerosols
- Air Microbiology
- Cough
(microbiology)
- DNA Fingerprinting
- DNA, Bacterial
(analysis, genetics)
- Environmental Monitoring
(methods, standards)
- Epidemiological Monitoring
- Feasibility Studies
- Female
- Humans
- Male
- Molecular Epidemiology
- Mycobacterium tuberculosis
(genetics, isolation & purification, pathogenicity)
- Particle Size
- Reproducibility of Results
- Selection Bias
- Sensitivity and Specificity
- Sputum
(microbiology)
- Tuberculosis, Multidrug-Resistant
(complications, drug therapy, epidemiology, microbiology, transmission)
- Tuberculosis, Pulmonary
(complications, drug therapy, epidemiology, microbiology, transmission)
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