Analysis of the RNA helicase A gene in human lung cancer.

Abstract

In this study we investigated the RNA helicase A (RHA) gene localized on chromosome 1q25 in 15 lung cancer cell lines, 54 primary carcinomas, bronchial epithelial cells and normal lung. Both RT-PCR and real-time RT-PCR showed that RHA is over-expressed in tumour samples compared to normal lung tissues (p=0.01). There was a tendency for higher expression levels in small cell lung cancer compared to non-small cell carcinomas. Over-expression of Y-162 correlated with high grade tumours (p=0.023). However, there was no correlation with tumour stage and survival. Our observations are compatible with the role of RHA in lung cancer development.

• Authors: Xiao Wei, Manuela Pacyna-Gengelbach, Karsten Schlüns, Qian An, Yanning Gao, Shujun Cheng, Iver Petersen
• Journal: Oncology reports (Oncol Rep) Vol. 11 Issue 1 Pg. 253-8 (Jan 2004) ISSN: 1021-335X [Print] Greece
• PMID: 14654934 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantigens
• Neoplasm Proteins
• RNA, Neoplasm
• DHX9 protein, human
• DEAD-box RNA Helicases
• RNA Helicases

Topics

• Autoantigens (genetics)
• Blotting, Northern
• Cell Line
• Cell Line, Tumor
• DEAD-box RNA Helicases
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (enzymology, genetics, pathology)
• Neoplasm Proteins
• Nucleic Acid Hybridization (methods)
• RNA Helicases (genetics)
• RNA, Neoplasm (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction