Abstract |
The Type II feline infectious peritonitis virus (FIPV) infection of feline macrophages is enhanced by a monoclonal antibody (MAb) to the S protein of FIPV. This antibody-dependent enhancement (ADE) activity increased with the MAb that showed a neutralizing activity with feline kidney cells, suggesting that there was a distinct correlation between ADE activity and the neutralizing activity. The close association between enhancing and neutralizing epitopes is an obstacle to developing a vaccine containing only neutralizing epitopes without enhancing epitopes. In this study, we immunized cats with cell lysate with recombinant baculovirus-expressed N protein of the Type I FIPV strain KU-2 with an adjuvant and investigated its preventive effect on the progression of FIP. Cats immunized with this vaccine produced antibodies against FIPV virion-derived N protein but did not produce virus- neutralizing antibodies. A delayed type hypersensitivity skin response to N protein was observed in these vaccinated cats, showing that cell mediated immunity against the FIPV antigen was induced. When these vaccinated cats were challenged with a high dose of heterologous FIPV, the survival rate was 75% (6/8), while the survival rate in the control group immunized with SF-9 cell-derived antigen was 12.5% (1/8). This study showed that immunization with the cell lysate with baculovirus-expressed N protein was effective in preventing the progression of FIP without inducing ADE of FIPV infection in cats.
|
Authors | Tsutomu Hohdatsu, Hiroshi Yamato, Tasuku Ohkawa, Miyuki Kaneko, Kenji Motokawa, Hajime Kusuhara, Takashi Kaneshima, Setsuo Arai, Hiroyuki Koyama |
Journal | Veterinary microbiology
(Vet Microbiol)
Vol. 97
Issue 1-2
Pg. 31-44
(Dec 02 2003)
ISSN: 0378-1135 [Print] Netherlands |
PMID | 14637036
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies, Viral
- Antigens, Viral
- Nucleocapsid Proteins
- Recombinant Proteins
- Viral Vaccines
|
Topics |
- Animals
- Antibodies, Viral
(blood)
- Antigens, Viral
(immunology)
- Baculoviridae
(genetics)
- Cats
- Coronavirus, Feline
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Feline Infectious Peritonitis
(immunology, prevention & control)
- Hypersensitivity, Delayed
(immunology)
- Neutralization Tests
(veterinary)
- Nucleocapsid Proteins
(genetics, immunology)
- Recombinant Proteins
(genetics, immunology)
- Sensitivity and Specificity
- Specific Pathogen-Free Organisms
- Vaccination
(veterinary)
- Viral Vaccines
(immunology, standards)
|