[Study on diagnosis of schistosomiasis by ELISA using periodate-treated soluble egg antigen].

Abstract

OBJECTIVE:

To improve SEA-ELISA, an immunodiagnostic assay for schistosomiasis.

METHODS:

Soluble egg antigen (SEA) of Schistosoma japonicum was treated with sodium periodate (SP) in order to oxidate its glycosylated epitopes. ELISA using the treated SEA was then performed to detect specific antibodies to SEA in the sera of schistosomiasis patients.

RESULTS:

Serum samples were tested by ELISA using SEA treated with sodium periodate (SP-SEA-ELISA), including 64 sera from cases with chronic schistosomiasis japonica, 119 sera from normal individuals in non-endemic area, 34 sera from patients with clonorchiasis, 33 sera of paragonimiasis cases and 36 sera from patients with cysticercosis. The results showed that its sensitivity (98.4%) was similar to that of the routine SEA-ELISA (100.0%) (P > 0.05) and the specificity is higher than that of the SEA-ELISA (P < 0.05). SP-SEA-ELISA showed a higher negative rate (89.0%) for sera of schistosomiasis patients 12 months post-treatment than that of the SEA-ELISA (42.1%).

CONCLUSION:

Use of SP-SEA can increase the specificity of ELISA, reduce cross-reactivity with serum samples from cases infected with other parasites and improve its value in evaluating therapeutic efficacy.

• Authors: Yue-long Huang, Xin-yuan Yi, Xian-fang Zeng, Ran Zhang, Shi-shan Yuan
• Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi) Vol. 21 Issue 4 Pg. 238-41 ( 2003) ISSN: 1000-7423 [Print] China
• PMID: 14628379 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Periodic Acid
• metaperiodate

Topics

• Animals
• Antibodies, Helminth (blood)
• Antibody Specificity
• Antigens, Helminth
• Enzyme-Linked Immunosorbent Assay
• Humans
• Periodic Acid
• Rabbits
• Schistosoma japonicum (immunology)
• Schistosomiasis japonica (diagnosis)
• Sensitivity and Specificity