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ATP-binding cassette transporters are enriched in non-caveolar detergent-insoluble glycosphingolipid-enriched membrane domains (DIGs) in human multidrug-resistant cancer cells.

Abstract
In this study we show that P-glycoprotein in multidrug-resistant 2780AD human ovarian carcinoma cells and multidrug resistance-associated protein 1 in multidrug-resistant HT29col human colon carcinoma cells are predominantly located in Lubrol-based detergent-insoluble glycosphingolipid-enriched membrane domains. This localization is independent of caveolae, since 2780AD cells do not express caveolin-1. Although HT29col cells do express caveolin-1, the ATP-binding cassette transporter and caveolin-1 were dissociated on the basis of differential solubility in Triton X-100 and absence of microscopical colocalization. While both the multidrug resistance-associated protein 1 and caveolin-1 are located in Lubrol-based membrane domains, they occupy different regions of these domains.
AuthorsJohn W J Hinrichs, Karin Klappe, Ina Hummel, Jan W Kok
JournalThe Journal of biological chemistry (J Biol Chem) Vol. 279 Issue 7 Pg. 5734-8 (Feb 13 2004) ISSN: 0021-9258 [Print] United States
PMID14627714 (Publication Type: Journal Article)
Chemical References
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • ATP-Binding Cassette Transporters
  • Chemokines, CC
  • Detergents
  • Glycosphingolipids
  • Ccl6 protein, mouse
  • Polyethylene Glycols
  • lubrol
  • Adenosine Triphosphate
  • Octoxynol
Topics
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 (biosynthesis)
  • ATP-Binding Cassette Transporters (chemistry, physiology)
  • Adenosine Triphosphate (chemistry)
  • Caveolae (metabolism)
  • Cell Line, Tumor
  • Cell Membrane (metabolism)
  • Chemokines, CC (biosynthesis)
  • Detergents (pharmacology)
  • Drug Resistance, Multiple
  • Glycosphingolipids (chemistry)
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Octoxynol (pharmacology)
  • Polyethylene Glycols (pharmacology)
  • Precipitin Tests
  • Protein Structure, Tertiary
  • Reverse Transcriptase Polymerase Chain Reaction

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