A natural musaceas plant extract inhibits proteasome activity and induces apoptosis selectively in human tumor and transformed, but not normal and non-transformed, cells.
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| Abstract |
Animal studies have demonstrated that a dietary polyphenol known as tannic acid (TA) exhibits anticarcinogenic activity in chemically induced cancers. Most recently, we have reported that TA and ester-bond containing green tea polyphenols are potent proteasome inhibitors in vitro and in vivo. We hypothesize that CellQuest, a patented formula which contains high level of TA obtained from a musaceas (plantain) plant extract, will inhibit the tumor cell proteasome activity. Here, we report that a partially purified CellQuest fraction, S3, potently inhibits the proteasomal chymotrypsin-like activity of Jurkat T cell extracts in a concentration-dependent manner. Inhibition of the proteasome by S3 in leukemia Jurkat T, simian virus 40-transformed and prostate cancer LNCaP cells results in accumulation of ubiquitinated proteins and the natural proteasome substrate p27Kip1, followed by induction of apoptosis. In contrast, non-transformed, immortalized human natural killer cells and normal human fibroblasts are resistant to S3-mediated proteasome inhibition and apoptosis induction. Our present study suggests that CellQuest targets and inhibits the proteasome selectively in tumor cells, which may contribute to the claimed anticancer activity.
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| Authors | Aslamuzzaman Kazi, Daniel A Urbizu, Deborah J Kuhn, Abelardo L Acebo, Edward R Jackson, Gail P Greenfelder, Nagi B Kumar, Q Ping Dou |
| Journal | International journal of molecular medicine (Int J Mol Med) Vol. 12 Issue 6 Pg. 879-87 (Dec 2003) ISSN: 1107-3756 [Print] Greece |
| PMID | 14612961 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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