[Comparison of auramine-rhodamine and Erlich-Ziehl-Neelsen staining methods for the diagnosis of tuberculosis].

Abstract

The aim of this study was to compare auramin-rhodamine (A-R) and Erlich-Ziehl-Neelsen (EZN) staining techniques for the diagnosis of tuberculosis. Of 311 sputum samples collected from active pulmonary tuberculosis patients and tuberculosis-suspected patients, 103 (33%) were found culture positive. In the direct microscopic examination of EZN stained smears, 86.4% (89/103) of culture positive samples, and 3.8% (8/208) of culture negative samples yielded asido-resistant bacteria, while these rates were 74.8% (77/103) and 11.5% (24/208) for A-R staining method. When culture was accepted as reference method, the specificity and sensitivity of the staining techniques were found as 88.5% and 74.8% for A-R, and 96.2% and 86.4% for EZN, respectively. As a result, it was concluded that, the use of A-R staining alone, could not be an alternative method to EZN staining.

• Authors: Gülnur Tarhan, Lale Ordulu, Feyzullah Gümüşlü, Ismail Ceyhan, Salih Cesur
• Journal: Mikrobiyoloji bulteni (Mikrobiyol Bul) 2003 Apr-Jun Vol. 37 Issue 2-3 Pg. 131-6 ISSN: 0374-9096 [Print] Turkey
• Vernacular Title: Tüberküloz tanisinda auramine-rhodamine ve Erlich-Ziehl-Neelsen boyama yöntemlerinin karşilaştirilmasi.
• PMID: 14593894 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Coloring Agents
• Fluorescent Dyes
• Rhodamines
• Benzophenoneidum

Topics

• Benzophenoneidum
• Coloring Agents
• Fluorescent Dyes
• Humans
• Mycobacterium tuberculosis (isolation & purification)
• Rhodamines
• Sensitivity and Specificity
• Sputum (microbiology)
• Staining and Labeling (methods)
• Tuberculosis, Pulmonary (diagnosis)