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Phosphocreatine restores high-energy phosphates in ischemic myocardium: implication for off-pump cardiac revascularization.

AbstractBACKGROUND:
High-energy phosphate metabolism is altered in the ischemic myocardium. We investigated the effects of in vivo administration of phosphocreatine (PCr) in a transient ischemic rat model to emulate off-pump myocardial revascularization.
STUDY DESIGN:
Rats received either PCr (100 mg/kg) or saline intravenously 1 hour before surgery. Regional ischemia was maintained for 12 minutes by ligation of the left anterior descending artery and compared with sham-operated animals. Cardiac tissue was studied for ATP, PCr, and inorganic phosphate (Pi) using (31)P-cryo-NMR. Results were compared by ANOVA.
RESULTS:
Levels of ATP were significantly (p < 0.01) lower in the ischemic hearts compared with controls; Pi and PCr remained unchanged. The PCr/Pi ratio was altered in ischemic hearts, reflecting an increased energy demand. PCr administration significantly (p < 0.01) elevated the content of PCr and ATP in both normal and ischemic hearts.
CONCLUSIONS:
PCr restores high-energy phosphates and attenuates metabolic stress during periods of myocardial ischemia in the rat. Preconditioning with PCr may serve as a useful adjunct to off-pump coronary revascularization.
AuthorsGanga Prabhakar, Linda Vona-Davis, David Murray, Paresh Lakhani, Gordon Murray
JournalJournal of the American College of Surgeons (J Am Coll Surg) Vol. 197 Issue 5 Pg. 786-91 (Nov 2003) ISSN: 1072-7515 [Print] United States
PMID14585415 (Publication Type: Journal Article)
Chemical References
  • Phosphates
  • Phosphorus Isotopes
  • Phosphocreatine
  • Adenosine Triphosphate
Topics
  • Adenosine Triphosphate (analysis, metabolism)
  • Analysis of Variance
  • Animals
  • Coronary Vessels (surgery)
  • Disease Models, Animal
  • Drug Evaluation, Preclinical
  • Energy Metabolism
  • Glycolysis (drug effects)
  • Injections, Intravenous
  • Ischemic Preconditioning, Myocardial (methods)
  • Ligation
  • Magnetic Resonance Imaging
  • Male
  • Myocardial Ischemia (diagnosis, drug therapy, metabolism)
  • Myocardial Reperfusion (methods)
  • Myocardium (chemistry, metabolism)
  • Phosphates (analysis, metabolism)
  • Phosphocreatine (pharmacology, therapeutic use)
  • Phosphorus Isotopes
  • Phosphorylation (drug effects)
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors

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