Abstract | BACKGROUND: STUDY DESIGN: Rats received either PCr (100 mg/kg) or saline intravenously 1 hour before surgery. Regional ischemia was maintained for 12 minutes by ligation of the left anterior descending artery and compared with sham-operated animals. Cardiac tissue was studied for ATP, PCr, and inorganic phosphate (Pi) using (31)P-cryo-NMR. Results were compared by ANOVA. RESULTS: Levels of ATP were significantly (p < 0.01) lower in the ischemic hearts compared with controls; Pi and PCr remained unchanged. The PCr/Pi ratio was altered in ischemic hearts, reflecting an increased energy demand. PCr administration significantly (p < 0.01) elevated the content of PCr and ATP in both normal and ischemic hearts. CONCLUSIONS: PCr restores high-energy phosphates and attenuates metabolic stress during periods of myocardial ischemia in the rat. Preconditioning with PCr may serve as a useful adjunct to off-pump coronary revascularization.
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Authors | Ganga Prabhakar, Linda Vona-Davis, David Murray, Paresh Lakhani, Gordon Murray |
Journal | Journal of the American College of Surgeons
(J Am Coll Surg)
Vol. 197
Issue 5
Pg. 786-91
(Nov 2003)
ISSN: 1072-7515 [Print] United States |
PMID | 14585415
(Publication Type: Journal Article)
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Chemical References |
- Phosphates
- Phosphorus Isotopes
- Phosphocreatine
- Adenosine Triphosphate
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Topics |
- Adenosine Triphosphate
(analysis, metabolism)
- Analysis of Variance
- Animals
- Coronary Vessels
(surgery)
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Energy Metabolism
- Glycolysis
(drug effects)
- Injections, Intravenous
- Ischemic Preconditioning, Myocardial
(methods)
- Ligation
- Magnetic Resonance Imaging
- Male
- Myocardial Ischemia
(diagnosis, drug therapy, metabolism)
- Myocardial Reperfusion
(methods)
- Myocardium
(chemistry, metabolism)
- Phosphates
(analysis, metabolism)
- Phosphocreatine
(pharmacology, therapeutic use)
- Phosphorus Isotopes
- Phosphorylation
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Time Factors
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