Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: No overall correlation between the extent of involucrin expression and pimonidazole binding was observed. The lack of correlation was because of heterogeneous patterns of immunostaining for involucrin generally related to tumor grade. Colocalized immunostaining for involucrin and pimonidazole binding was observed in intermediate grade tumors but not in well-differentiated or poorly differentiated tumors. Human MT-IIa mRNA and MT protein were expressed in basal lamina of normal human epithelia and in the proliferative rims of tumor nests. CONCLUSIONS: Colocalization of immunostaining for involucrin and pimonidazole binding is consistent with oxygen regulation, but the lack of involucrin expression in hypoxic regions of poorly differentiated tumors indicates that its transcriptional status with respect to hypoxia induction is altered by cell differentiation. The localization of MT message and protein in the outer rims of most tumor nests indicates that the transcriptional status of metallothionein is also altered by differentiation.
|
Authors | Yoshihiro Azuma, Shu-Chuan Chou, Ruth A Lininger, Brian J Murphy, Mahesh A Varia, James A Raleigh |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 9
Issue 13
Pg. 4944-52
(Oct 15 2003)
ISSN: 1078-0432 [Print] United States |
PMID | 14581369
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- MT2A protein, human
- Nitroimidazoles
- Protein Precursors
- RNA, Messenger
- Radiation-Sensitizing Agents
- pimonidazole
- involucrin
- Metallothionein
- Oxygen
|
Topics |
- Biopsy
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Cell Differentiation
- Cell Line, Tumor
- Female
- Humans
- Hypoxia
- Immunohistochemistry
- In Situ Hybridization
- Metallothionein
(biosynthesis)
- Nitroimidazoles
(pharmacology)
- Oxygen
(metabolism)
- Prognosis
- Protein Precursors
(pharmacology)
- RNA, Messenger
(metabolism)
- Radiation-Sensitizing Agents
(pharmacology)
- Transcription, Genetic
- Uterine Cervical Neoplasms
(diagnosis, pathology)
|