HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Zoledronic acid inhibits osteoclastogenesis in vitro and in a mouse model of inflammatory osteolysis.

Abstract
This study assessed effects of the bisphosphonate zoledronic acid (ZLNA) on osteoclastogenesis. To assess the effect of ZLNA on osteoclast formation in vitro, we cultured mouse bone marrow cells under conditions that promote osteoclastogenesis. Administered at concentrations from 10(-6) to 10(-9) mol/L, ZLNA led to a dose-dependent inhibition of osteoclastogenesis. Combined TUNEL staining and histochemical staining for tartrate-resistant acid phosphatase showed that ZLNA induced apoptosis in osteoclasts and monocytic precursor cells. To study the effects of ZLNA in vivo, we placed keratin particles onto the surface of the parietal bone of mice to induce localized inflammatory bone resorption. Three experimental groups received daily subcutaneous injections of ZLNA (1, 3, or 10 microg/kg body weight) from 4 days before surgery until 5 days after keratin implantation. The ZLNA significantly reduced osteoclast recruitment in a dose-dependent manner, but did not affect the degree of inflammation or the mineral apposition rate.
AuthorsHolger Sudhoff, Jae Y Jung, Jörg Ebmeyer, Brian T Faddis, Henning Hildmann, Richard A Chole
JournalThe Annals of otology, rhinology, and laryngology (Ann Otol Rhinol Laryngol) Vol. 112 Issue 9 Pt 1 Pg. 780-6 (Sep 2003) ISSN: 0003-4894 [Print] United States
PMID14535562 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Diphosphonates
  • Imidazoles
  • Minerals
  • Keratins
  • Zoledronic Acid
Topics
  • Animals
  • Bone Marrow Cells (pathology)
  • Bone Resorption (etiology)
  • Cell Division (drug effects)
  • Cells, Cultured
  • Diphosphonates (administration & dosage, pharmacology)
  • Dose-Response Relationship, Drug
  • Imidazoles (administration & dosage, pharmacology)
  • Injections, Subcutaneous
  • Keratins (adverse effects)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Minerals (metabolism)
  • Osteitis (complications)
  • Osteoclasts (pathology)
  • Osteolysis (etiology, pathology)
  • Parietal Bone (pathology, surgery)
  • Periosteum (metabolism)
  • Prostheses and Implants (adverse effects)
  • Skull (metabolism)
  • Zoledronic Acid

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: