Proteasome inhibitor (MG-132) treatment of mdx mice rescues the expression and membrane localization of dystrophin and dystrophin-associated proteins.

Abstract

Dystrophin, the protein product of the Duchenne muscular dystrophy (DMD) gene, is absent in the skeletal muscle of DMD patients and mdx mice. At the plasma membrane of skeletal muscle fibers, dystrophin associates with a multimeric protein complex, termed the dystrophin-glycoprotein complex (DGC). Protein members of this complex are normally absent or greatly reduced in dystrophin-deficient skeletal muscle fibers, and are thought to undergo degradation through an unknown pathway. As such, we reasoned that inhibition of the proteasomal degradation pathway might rescue the expression and subcellular localization of dystrophin-associated proteins. To test this hypothesis, we treated mdx mice with the well-characterized proteasomal inhibitor MG-132. First, we locally injected MG-132 into the gastrocnemius muscle, and observed the outcome after 24 hours. Next, we performed systemic treatment using an osmotic pump that allowed us to deliver different concentrations of the proteasomal inhibitor, over an 8-day period. By immunofluorescence and Western blot analysis, we show that administration of the proteasomal inhibitor MG-132 effectively rescues the expression levels and plasma membrane localization of dystrophin, beta-dystroglycan, alpha-dystroglycan, and alpha-sarcoglycan in skeletal muscle fibers from mdx mice. Furthermore, we show that systemic treatment with the proteasomal inhibitor 1) reduces muscle membrane damage, as revealed by vital staining (with Evans blue dye) of the diaphragm and gastrocnemius muscle isolated from treated mdx mice, and 2) ameliorates the histopathological signs of muscular dystrophy, as judged by hematoxylin and eosin staining of muscle biopsies taken from treated mdx mice. Thus, the current study opens new and important avenues in our understanding of the pathogenesis of DMD. Most importantly, these new findings may have clinical implications for the pharmacological treatment of patients with DMD.

• Authors: Gloria Bonuccelli, Federica Sotgia, William Schubert, David S Park, Philippe G Frank, Scott E Woodman, Luigi Insabato, Michael Cammer, Carlo Minetti, Michael P Lisanti
• Journal: The American journal of pathology (Am J Pathol) Vol. 163 Issue 4 Pg. 1663-75 (Oct 2003) ISSN: 0002-9440 [Print] United States
• PMID: 14507673 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cysteine Proteinase Inhibitors
• Cytoskeletal Proteins
• Dystrophin
• Leupeptins
• Membrane Glycoproteins
• Sarcoglycans
• Dystroglycans
• benzyloxycarbonylleucyl-leucyl-leucine aldehyde

Topics

• Animals
• Cell Membrane (metabolism)
• Cysteine Proteinase Inhibitors (administration & dosage, pharmacology)
• Cytoskeletal Proteins (metabolism)
• Drug Administration Schedule
• Dystroglycans
• Dystrophin (metabolism)
• Infusion Pumps
• Injections, Intramuscular
• Injections, Subcutaneous
• Leupeptins (administration & dosage, pharmacology)
• Membrane Glycoproteins (metabolism)
• Mice
• Mice, Inbred mdx
• Muscle, Skeletal (metabolism, pathology)
• Muscular Dystrophy, Duchenne (metabolism, pathology)
• Sarcoglycans
• Tissue Distribution