In vitro secretion of transforming growth factor alpha (TGF-alpha): a comparison of the A431 cell line with three human oesophageal squamous cell carcinoma lines.

Abstract

Transforming growth factor alpha (TGF-alpha) is a single chain polypeptide which exists in a variety of forms differing in molecular weight. These forms are variously present in normal and neoplastic cells. Of particular interest are TGF-alpha's well-known mitogenic properties. The transition from a normal to a neoplastic cellular state results from signalling defects that may depend upon, inter alia, abnormal levels of expression and secretion of TGF-alpha. It is known that the secretion of TGF-alpha may be enhanced appreciably by agents such as phorbol 12-myristate 13-acetate (PMA), serum factors and epidermal growth factor (EGF). Here, we compare the efficacy of these three agents in the elevation of TGF-alpha secretion in the well studied A431 cell line with their previously undocumented efficacy in certain interesting, but little known, human oesophageal squamous cell carcinoma (SCC) lines.

• Authors: A L Thornley, G J Jones
• Journal: Bioscience reports (Biosci Rep) Vol. 12 Issue 4 Pg. 293-302 (Aug 1992) ISSN: 0144-8463 [Print] England
• PMID: 1421058 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Transforming Growth Factor alpha
• Epidermal Growth Factor
• ErbB Receptors
• Tetradecanoylphorbol Acetate

Topics

• Carcinoma, Squamous Cell (metabolism)
• Epidermal Growth Factor (pharmacology)
• ErbB Receptors (physiology)
• Esophageal Neoplasms (metabolism)
• Humans
• In Vitro Techniques
• Secretory Rate (drug effects)
• Tetradecanoylphorbol Acetate (pharmacology)
• Transforming Growth Factor alpha (metabolism)
• Tumor Cells, Cultured (metabolism)