Abstract |
A 3789 nucleotide region of the bovine ephemeral fever virus (BEFV) genome, located 1.65 kb downstream of the N gene, has been cloned and sequenced. The region contains two long open reading frames (ORFs) which are bounded by putative consensus (AACAGG) and polyadenylation (CATG[ A]7) sequences and are separated by an intergenic region of 53 nucleotides. Discrete mRNAs corresponding to each ORF have been identified. The first ORF encodes a polypeptide comprising 623 residues which was identified by peptide sequencing as the virion G protein. The deduced amino acid sequence of the G protein includes putative signal and transmembrane domains and five potential glycosylation sites. The second ORF encodes a polypeptide of 586 amino acids which also has characteristics of a rhabdovirus glycoprotein, including putative signal and transmembrane domains and eight potential glycosylation sites, and appears to correspond to a 90-kDa nonstructural glycoprotein (GNS) identified in BEFV-infected cells (Walker et al. [1991] J. Gen. Virol. 72, 67-74). A database search indicated that both the G and GNS proteins share significant amino acid sequence homology with other rhabdovirus G proteins and with each other. Highest homology scores for each protein were with sigma virus and vesicular stomatitis virus serotypes.
|
Authors | P J Walker, K A Byrne, G A Riding, J A Cowley, Y Wang, S McWilliam |
Journal | Virology
(Virology)
Vol. 191
Issue 1
Pg. 49-61
(Nov 1992)
ISSN: 0042-6822 [Print] United States |
PMID | 1413521
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- DNA, Viral
- Glycoproteins
- RNA, Messenger
- Viral Nonstructural Proteins
- Viral Proteins
- BEFV G protein, Bovine ephemeral fever virus
- GNS protein, Bovine ephemeral fever virus
|
Topics |
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Viral
- Genome, Viral
- Glycoproteins
(genetics, isolation & purification)
- Molecular Sequence Data
- RNA, Messenger
- Rhabdoviridae
(genetics)
- Sequence Homology, Amino Acid
- Viral Nonstructural Proteins
- Viral Proteins
(genetics, isolation & purification)
|