Abstract |
Serum from C3H/He mice, which show regression of MM2 tumor cells after transplantation and removal (regressor serum, RS) contains non-gammaglobulin components that cross-react with various tumor cells of mice [22, 23]. In addition to tumor cells, various allogeneic lymphocytes are also susceptible to an RS-dependent lymphocyte-mediated cytotoxic reaction. To identify tumor cell surface antigens that cause the cross-reactive host response, the serum components were analyzed by absorption of RS with allogeneic lymphocytes. RS components were found to recognize allogeneic lymphocyte antigens including Qa-2 and Ly6.2. Specificity for the Qa-2 antigen was further tested using Qa-2-congenic mice. The expression of Qa-2 antigen was detected on the surfaces of MM2 and other tumor cells derived from H-2k mice (seven among nine cell lines tested) by a membrane immunofluorescence method using a Qa-2-specific mAb. Physical characteristics of the Qa-2-specific component in RS were determined and found to differ from those of regular IgGs but to be similar to those of IgDs. Using an enzyme-linked immunosorbent assay with an IgD-specific mAb and Qa-2-lacZ fusion protein, the existence of IgD in RS with specificity for Qa-2 was confirmed. These results suggest that the RS component with Qa-2 specificity is an IgD, the specificity and physiological role of which are unknown.
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Authors | T Tanino, N Seo, T Okazaki, C Nakanishi-Ito, M Sekimata, K Egawa |
Journal | Cancer immunology, immunotherapy : CII
(Cancer Immunol Immunother)
Vol. 35
Issue 4
Pg. 230-6
( 1992)
ISSN: 0340-7004 [Print] Germany |
PMID | 1380893
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Ly
- Antigens, Neoplasm
- Epitopes
- Histocompatibility Antigens Class I
- Immunoglobulin D
- Isoantigens
- Membrane Glycoproteins
- Q surface antigens
- thymus-leukemia antigens
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Topics |
- Animals
- Antigens, Ly
- Antigens, Neoplasm
(analysis, immunology)
- Cross Reactions
(immunology)
- Epitopes
- Flow Cytometry
- Histocompatibility Antigens Class I
(analysis, immunology)
- Immunoglobulin D
(analysis)
- Isoantigens
(analysis, immunology)
- Membrane Glycoproteins
(analysis, immunology)
- Mice
- Mice, Inbred Strains
- Neoplasms, Experimental
(blood, immunology)
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