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Schedule of spermatogenesis in the pulmonate snail Helix aspersa, with special reference to histone transition.

Abstract
The schedule of spermatogenesis is determined from the times necessary for cells labeled with tritium thymidine during premeiotic DNA synthesis to pass through the successive spermatogenic stages. A transition from a typically somatic histone rich in lysine, to a histone rich in arginine is shown to occur during spermatid stages. A later shift to a protamine is observed in the maturing sperm. These changes are characterized by the use of in situ staining methods. The transition to an arginine-rich histone is accompanied by incorporation of tritium-labeled arginine, hence reflects synthesis of new protein. Comparison of the timing of arginine and thymidine incorporation, and independent measurements of DNA, show that in contrast to the case of premitotic chromosome duplication, the histone synthesis in the spermatid is unaccompanied by DNA synthesis. During the initial histone change, fine filaments are formed within the nucleus, which aggregate to form lamellae. This fine structure is lost during maturation of the sperm.
AuthorsD P BLOCH, H Y HEW
JournalThe Journal of biophysical and biochemical cytology (J Biophys Biochem Cytol) Vol. 7 Pg. 515-32 (Jun 1960) ISSN: 0095-9901 [Print] United States
PMID13801496 (Publication Type: Journal Article)
Chemical References
  • Histones
  • Protamines
  • Tritium
  • DNA
  • Arginine
  • Lysine
  • Thymidine
Topics
  • Arginine
  • Cell Nucleus
  • DNA
  • Histones (chemistry)
  • Humans
  • Lysine
  • Male
  • Protamines
  • Snails
  • Sperm Maturation
  • Spermatids
  • Spermatogenesis
  • Spermatozoa
  • Staining and Labeling
  • Thymidine
  • Tritium

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