[Immunohistological investigation of epithelial components of adenolymphoma of the parotid gland].

Abstract

In order to develop a more objective method of evaluating the origin of adenolymphoma, we immunohistochemically investigated the expression of cytokeratins, vimentin, S-100 protein, and alpha-smooth-muscle actin using the avidin-biotin-peroxidase (ABC) method in ethanol-fixed paraffin-embedded specimens from 8 adenolymphomas. Several kinds of monoclonal antibodies which react monospecifically with each subclass of cytokeratins were used. Results were compared with specimens of 8 normal parotid glands by radical neck dissection in patients with other diseases who had not undergone radiotherapy. In the adenolymphoma specimens, basal cells were strongly positive for CK-6, but reactivity of columnar cells was apparently reduced. In contrast, columnar cells were strongly positive for CK-7, but reactivity of basal cells was reduced. In normal parotid gland specimens, CK-7 was also detected in all columnar cells in the ductal system, although some duct cells around the columnar cells, which showed strong CK-6 expression, showed poor CK-7 expression. alpha-smooth-muscle actin was present in myoepithelial cells in normal parotid gland specimens, but not in basal cells of adenolymphoma or of the normal ductal system, which were CK-6-positive. As these characteristic findings were consistently observed in all specimens, the demonstration of CK-6, CK-7, and alpha-smooth-muscle actin may be useful in the recognition and classification of columnar and basal cells. CK-8 was present in both columnar and basal cells of adenolymphoma and of the normal duct, but in the normal parotid gland, acinus cells were also strongly positive for CK-8.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: M Murakami, I Makino, Y Nishiyama, S Mimaki, Y Murakami
• Journal: Nihon Jibiinkoka Gakkai kaiho (Nihon Jibiinkoka Gakkai Kaiho) Vol. 95 Issue 4 Pg. 526-34 (Apr 1992) ISSN: 0030-6622 [Print] Japan
• PMID: 1376356 (Publication Type: English Abstract, Journal Article)

Chemical References

• Actins
• S100 Proteins
• Vimentin
• Keratins

Topics

• Actins (metabolism)
• Adenolymphoma (metabolism)
• Epithelium (metabolism)
• Female
• Humans
• Immunohistochemistry
• Keratins (metabolism)
• Male
• Parotid Gland (metabolism)
• Parotid Neoplasms (metabolism)
• S100 Proteins (metabolism)
• Vimentin (metabolism)