Abstract |
In this study, we show that the Saccharomyces cerevisiae ORF YBR142w, which encodes a putative DEAD-box RNA helicase, corresponds to MAK5. The mak5-1 allele is deficient in the maintenance of the M1 dsRNA virus, resulting in a killer minus phenotype. This allele carries two mutations, G218D in the conserved ATPase A-motif and P618S in a non-conserved region. We have separated these mutations and shown that it is the G218D mutation that is responsible for the killer minus phenotype. Mak5p is an essential nucleolar protein; depletion of the protein leads to a reduction in the level of 60S ribosomal subunits, the appearance of half-mer polysomes, and a delay in production of the mature 25S and 5.8S rRNAs. Thus, Mak5p is involved in the biogenesis of 60S ribosomal subunits.
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Authors | M Zagulski, D Kressler, A-M Bécam, J Rytka, C J Herbert |
Journal | Molecular genetics and genomics : MGG
(Mol Genet Genomics)
Vol. 270
Issue 3
Pg. 216-24
(Nov 2003)
ISSN: 1617-4615 [Print] Germany |
PMID | 13680366
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Primers
- RNA, Double-Stranded
- Recombinant Proteins
- Saccharomyces cerevisiae Proteins
- MAK5 protein, S cerevisiae
- DEAD-box RNA Helicases
- RNA Helicases
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Topics |
- Amino Acid Substitution
- Base Sequence
- DEAD-box RNA Helicases
- DNA Primers
- Gene Deletion
- Genetic Complementation Test
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Open Reading Frames
(genetics)
- Phenotype
- Plasmids
(genetics)
- Polymerase Chain Reaction
- RNA Helicases
(genetics, metabolism)
- RNA, Double-Stranded
(genetics)
- Recombinant Proteins
(metabolism)
- Ribosomes
(genetics, ultrastructure)
- Saccharomyces cerevisiae
(genetics)
- Saccharomyces cerevisiae Proteins
(genetics)
- Transcription, Genetic
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