Reciprocal alterations of GMP reductase and IMP dehydrogenase activities during differentiation in HL-60 leukemia cells.

Abstract

The study was undertaken to elucidate the regulatory roles of GMP reductase (GMPR) and IMP dehydrogenase (IMPDH) on purine interconversion during differentiation. Treatment of HL-60 cells with retinoic acid (1 microM) induced granulocytic differentiation which was accompanied with a 2.4-fold increase in GMPR and 55% decrease in IMPDH activities. Maturation induced by 12-O-tetradecanoylphorbol 13-acetate or dimethylsulfoxide was also associated with similar reciprocal alterations. Incubation with guanosine (200 microM), which expands the guanine nucleotide pool, elevated GMPR (1.9-fold) and decreased IMPDH (73%) activities. The synchronous and opposing alterations in GMPR and IMPDH activities should amplify the metabolic response due to differentiation or guanylate pool expansion.

• Authors: H Nakamura, Y Natsumeda, M Nagai, J Takahara, S Irino, G Weber
• Journal: Leukemia research (Leuk Res) 1992 Jun-Jul Vol. 16 Issue 6-7 Pg. 561-4 ISSN: 0145-2126 [Print] England
• PMID: 1353130 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Adenine Nucleotides
• Guanine Nucleotides
• IMP Dehydrogenase
• NADH, NADPH Oxidoreductases
• GMP Reductase

Topics

• Adenine Nucleotides (metabolism)
• Cell Differentiation
• GMP Reductase
• Guanine Nucleotides (metabolism)
• Humans
• IMP Dehydrogenase (metabolism)
• Leukemia, Promyelocytic, Acute (enzymology, metabolism, pathology)
• NADH, NADPH Oxidoreductases (metabolism)
• Tumor Cells, Cultured (enzymology, metabolism, pathology)