Reciprocal alterations of GMP reductase and IMP dehydrogenase activities during differentiation in HL-60 leukemia cells.

The study was undertaken to elucidate the regulatory roles of GMP reductase (GMPR) and IMP dehydrogenase (IMPDH) on purine interconversion during differentiation. Treatment of HL-60 cells with retinoic acid (1 microM) induced granulocytic differentiation which was accompanied with a 2.4-fold increase in GMPR and 55% decrease in IMPDH activities. Maturation induced by 12-O-tetradecanoylphorbol 13-acetate or dimethylsulfoxide was also associated with similar reciprocal alterations. Incubation with guanosine (200 microM), which expands the guanine nucleotide pool, elevated GMPR (1.9-fold) and decreased IMPDH (73%) activities. The synchronous and opposing alterations in GMPR and IMPDH activities should amplify the metabolic response due to differentiation or guanylate pool expansion.
AuthorsH Nakamura, Y Natsumeda, M Nagai, J Takahara, S Irino, G Weber
JournalLeukemia research (Leuk Res) 1992 Jun-Jul Vol. 16 Issue 6-7 Pg. 561-4 ISSN: 0145-2126 [Print] ENGLAND
PMID1353130 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Adenine Nucleotides
  • Guanine Nucleotides
  • IMP Dehydrogenase
  • NADH, NADPH Oxidoreductases
  • GMP Reductase
  • Adenine Nucleotides (metabolism)
  • Cell Differentiation
  • GMP Reductase
  • Guanine Nucleotides (metabolism)
  • Humans
  • IMP Dehydrogenase (metabolism)
  • Leukemia, Promyelocytic, Acute (enzymology, metabolism, pathology)
  • NADH, NADPH Oxidoreductases (metabolism)
  • Tumor Cells, Cultured (enzymology, metabolism, pathology)

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