Carbachol-stimulated calcium entry in SH-SY5Y human neuroblastoma cells: which route?

Abstract

M3 muscarinic receptors expressed on SH-SY5Y human neuroblastoma cells are linked to phosphoinositide turnover and rises in [Ca2+]i. The rise in [Ca2+]i is biphasic with the peak phase being due to release from an intracellular Ins(1,4,5)P3-sensitive site and the plateau phase being due to Ca2+ entry across the plasma membrane. Ca2+ entry does not appear to involve voltage sensitive Ca2+ channels, a pertussis toxin sensitive G-protein-operated Ca2+ channel or Ins(1,4,5)P3/Ins(1,3,4,5)P4-operated Ca2+ channel. We suggest that carbachol-stimulated Ca2+ entry in SH-SY5Y human neuroblastoma cells occurs via receptor operated Ca2+ channels and through capacitive refilling.

• Authors: D G Lambert, S R Nahorski
• Journal: Journal of physiology, Paris (J Physiol Paris) Vol. 86 Issue 1-3 Pg. 77-82 ( 1992) ISSN: 0928-4257 [Print] France
• PMID: 1343598 (Publication Type: Journal Article, Review)

Chemical References

• Calcium Channels
• Inositol Phosphates
• Phosphatidylinositols
• Receptors, Muscarinic
• Virulence Factors, Bordetella
• Carbachol
• Pertussis Toxin
• GTP-Binding Proteins
• Calcium

Topics

• Calcium (metabolism)
• Calcium Channels (physiology)
• Carbachol (pharmacology)
• Electrophysiology
• GTP-Binding Proteins (physiology)
• Humans
• Inositol Phosphates (physiology)
• Intracellular Membranes (metabolism)
• Neuroblastoma (metabolism, pathology)
• Osmolar Concentration
• Pertussis Toxin
• Phosphatidylinositols (metabolism)
• Receptors, Muscarinic (physiology)
• Tumor Cells, Cultured
• Virulence Factors, Bordetella (pharmacology)