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In situ localization of interleukin-6 in normal skin and atrophic cutaneous disease.

Abstract
Interleukin-6 (IL-6) is a multifunctional cytokine that participates in the inflammatory and immune responses. In human skin, keratinocytes produces IL-6, although the in vivo role of this cytokine is unknown. In the present study we investigated the in situ localization of IL-6 in normal epidermis (n = 10) and in a group of skin diseases characterized by epidermal atrophy. Formalin-fixed paraffin-embedded skin biopsies from patients with clinical and histopathological features consistent with localized scleroderma (n = 10), systemic scleroderma (n = 5), lichen sclerosus et atrophicus (n = 9) and balanitis xerotica obliterans (n = 7) were tested using polyclonal antibodies and avidin-biotin-peroxidase immunostaining. We demonstrated the presence of IL-6 in normal epidermis and in atrophic skin diseases. In normal skin there was moderate intercellular and intracellular reactivity detected using a high antibody concentration. In specimens with epidermal atrophy we detected intense cytoplasmic and intercellular immunostaining using a lower antibody concentration. The immunoreactivity was independent of the epidermal thickness. Plasma IL-6, measured by radioimmunoassay, was not elevated in plasma from patients with localized or systemic scleroderma. Increased IL-6 in the epidermis of selected skin diseases suggests that IL-6 may be related to the pathophysiology of dermatologic diseases characterized by epidermal atrophy.
AuthorsL I Romero, S H Pincus
JournalInternational archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 99 Issue 1 Pg. 44-9 ( 1992) ISSN: 1018-2438 [Print] SWITZERLAND
PMID1343091 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Autoantibodies
  • Interleukin-6
Topics
  • Antibody Specificity
  • Atrophy
  • Autoantibodies (analysis)
  • Balanitis (immunology)
  • Epidermis (immunology, pathology)
  • Humans
  • Immunoenzyme Techniques
  • Interleukin-6 (analysis)
  • Lichenoid Eruptions (immunology)
  • Male
  • Radioimmunoassay
  • Scleroderma, Localized (immunology)
  • Scleroderma, Systemic (immunology)
  • Skin (immunology)
  • Skin Diseases (immunology)

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