Abstract |
In this study we describe the isolation of genomic clones of the 5' region of the porcine ryanodine receptor gene, a candidate for malignant hyperthermia in pigs and humans. The recombinants were isolated from a porcine liver, genomic DNA library in phage EMBL3A after screening with PCR amplified DNA fragments. The exon/intron structure of the ryanodine receptor gene was determined by DNA sequencing. Based on the sequence data it was possible to develop a simple test for the detection of malignant hyperthermia susceptible and normal pigs.
|
Authors | B Brenig, G Brem |
Journal | FEBS letters
(FEBS Lett)
Vol. 298
Issue 2-3
Pg. 277-9
(Feb 24 1992)
ISSN: 0014-5793 [Print] England |
PMID | 1312025
(Publication Type: Journal Article)
|
Chemical References |
- Calcium Channels
- Receptors, Cholinergic
- Ryanodine Receptor Calcium Release Channel
- DNA
|
Topics |
- Animals
- Base Sequence
- Calcium Channels
(genetics, metabolism)
- DNA
- Humans
- Malignant Hyperthermia
(genetics, veterinary)
- Molecular Sequence Data
- Polymerase Chain Reaction
(veterinary)
- Receptors, Cholinergic
(genetics, metabolism)
- Restriction Mapping
- Ryanodine Receptor Calcium Release Channel
- Sequence Homology, Nucleic Acid
- Swine
- Swine Diseases
(genetics)
|