Abstract |
The presence of growth substance(s) for smooth muscle cells and fibroblasts was documented in extracts of human leiomyoma tissues (Koutsilieris et al, Am. J. Obst. Gynecol. 163: 1665-1670, 1990). In this study leiomyoma tissues (700 grs) obtained at surgery were extracted and purified using CM- sepharose, heparin-sepharose and reverse-phase high performance liquid chromatography (r-HPLC). The mouse NIH/3T3 cell fibroblasts (ATCC-CRL 1658) and the rat smooth muscle cells A10 (ATCC-CRL 1476) were used as indicator bioassay systems assessing the proliferative effect (tritiated- thymidine incorporation and cell number) of leiomyoma extracts. The profile of peptides purified by cm- sepharose, heparin-sepharose and by r-HPLC was analyzed by standard SDS gel electrophoresis. An overall 50,000 fold purification was achieved of a preferentially acting material on rat aorta smooth muscle cells ( A10 cells). This material possessed mitogenic activity equivalent to epidermal growth factor ( EGF) on A10 smooth muscle cells, in vitro. We believe that the final chemical definition of such leiomyoma-derived growth substance with preferential action on smooth muscle cells may elucidate the paracrine and/or autocrine interactions among myometrial cells and thus could provide new clues for the pathogenesis of uterine leiomyomas.
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Authors | M Koutsilieris, D Elmeliani, G Frenette, R Maheux |
Journal | In vivo (Athens, Greece)
(In Vivo)
1992 Nov-Dec
Vol. 6
Issue 6
Pg. 579-85
ISSN: 0258-851X [Print] Greece |
PMID | 1296805
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Growth Substances
- Mitogens
- Neoplasm Proteins
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Topics |
- 3T3 Cells
(drug effects)
- Animals
- Cell Division
(drug effects)
- Female
- Growth Substances
(isolation & purification, pharmacology)
- Humans
- Leiomyoma
(chemistry)
- Mice
- Mitogens
(isolation & purification, pharmacology)
- Muscle, Smooth
(drug effects)
- Neoplasm Proteins
(isolation & purification, pharmacology)
- Rats
- Uterine Neoplasms
(chemistry)
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