Gene expression and antitumor effect following im electroporation delivery of human interferon alpha 2 gene.

Abstract

AIM:

To investigate the gene expression and antitumor effect following im electroporation delivery of human interferon alpha 2 (hIFN-alpha 2) gene.

METHODS:

The pcD2/hIFN-alpha 2 was injected into the middle of the quadriceps muscle of female BALB/c mice or the leukemia-bearing female BALB/c nude mice, and then electroporation was given to the injection site. Optimal electrical parameters and the efficiency of gene transfer was studied with hIFN-alpha 2 ELISA kit. The HL-60 tumor model in BALB/c nude mice was used to investigate therapeutic effects of im electroporation delivery of pcD2/hIFN-alpha 2.

RESULTS:

The optimal conditions for the electric pulses were as follows: voltage at 200 V/cm; pulse duration at 40 ms per pulse; number of pulse at 6 pulses and frequency at 1 Hz. Under optimal conditions, the serum hIFN-alpha 2 levels in electroporation group (160 microg/L+/-31 microg/L) were 45-fold higher than those of nonelectroporation group (3.6 microg/L+/-1.6 microg/L, P<0.01). The growth of leukemia was inhibited more obviously and the survival time of the leukemia-bearing nude mice was prolonged after im electroporation delivery of pcD2/hIFN-alpha 2 100 microg or 200 microg.

CONCLUSION:

Electroporation was an efficient method for the delivery of plasmid DNA and im electroporation delivery of pcD2/hIFN-alpha 2 was effective in treating leukemia.

• Authors: Guo-Hua Zhang, Xiao-Fan Tan, Dong Shen, Shu-Yuan Zhao, Yan-Li Shi, Cai-Ke Jin, Wei-Gu Sun, Yan-Hong Guo, Kuang-Hueih Chen, Jian Tang
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 24 Issue 9 Pg. 891-6 (Sep 2003) ISSN: 1671-4083 [Print] United States
• PMID: 12956937 (Publication Type: Journal Article)

Chemical References

• Interferon-alpha

Topics

• Animals
• DNA Fragmentation
• Disease Models, Animal
• Electroporation
• Female
• Gene Transfer Techniques
• Genetic Therapy
• HL-60 Cells
• Humans
• Interferon-alpha (biosynthesis, blood, genetics, therapeutic use)
• Leukemia, Promyelocytic, Acute (therapy)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Muscle, Skeletal (metabolism)
• Neoplasm Transplantation
• Plasmids (genetics)