Abstract |
Oligodendrocyte-myelin glycoprotein (OMgp) is expressed on the surface of oligodendrocytes and neurones and is thought to inhibit axonal regeneration after brain injury in adult, like Nogo and myelin-associated glycoprotein (MAG). We previously observed that the OMgp gene locus on chromosome 17 could be associated with autism, a developmental disorder. The aim of the present study was to characterise the developmental expression of OMgp mRNA in the central nervous system. First we determined the rat OMgp gene sequence and compared it with the human and mouse sequences. Several regions, putative sites for the fixation of transcription factors, are conserved between these three species in the unique intron of this gene. Using quantitative and semi-quantitative RT-PCR, we studied OMgp gene expression in rat brain during post-natal development. We found that OMgp mRNA expression was developmentally regulated, with a peak of expression in the late stages of myelination. We observed a similar profile in oligodendrocyte cultures, in absence of neurones, suggesting that OMgp mRNA expression by oligodendrocytes was independent of axonal influence. Our observations suggest that OMgp is a late marker of myelination, which could be implicated in the arrest of oligodendrocyte proliferation, arrest of myelination or compaction of myelin.
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Authors | Patrick Vourc'h, Sabine Dessay, Olivier Mbarek, Sylviane Marouillat Védrine, Jean-Pierre Müh, Christian Andres |
Journal | Brain research. Developmental brain research
(Brain Res Dev Brain Res)
Vol. 144
Issue 2
Pg. 159-68
(Sep 10 2003)
ISSN: 0165-3806 [Print] Netherlands |
PMID | 12935913
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Complementary
- GPI-Linked Proteins
- MOG protein, human
- Mog protein, mouse
- Mog protein, rat
- Myelin Proteins
- Myelin-Associated Glycoprotein
- Myelin-Oligodendrocyte Glycoprotein
- OMG protein, human
- Omg protein, mouse
- Omg protein, rat
- RNA, Messenger
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Topics |
- Aging
- Amino Acid Sequence
- Animals
- Animals, Newborn
- Base Sequence
- Cells, Cultured
- Central Nervous System
(anatomy & histology, growth & development, metabolism)
- DNA, Complementary
(analysis)
- GPI-Linked Proteins
- Gene Expression Regulation, Developmental
- Humans
- Mice
- Molecular Sequence Data
- Myelin Proteins
- Myelin Sheath
(metabolism)
- Myelin-Associated Glycoprotein
(genetics)
- Myelin-Oligodendrocyte Glycoprotein
- Neuroglia
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Sequence Analysis
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