Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents.

In the process of screening for pancreatic lipase inhibitors, which could be used as an anti-obesity measure, the methanol extract of Dioscorea nipponica Makino powder (DP) appeared to have potent inhibitory activity against porcine pancreatic lipase with an IC50 value of 5-10 microg/ml, where the enzyme activity was assayed by using 4-methylumbelliferyl oleate as a substrate. Further purification of active components present in the herb generated dioscin that belongs to the saponin family. Dioscin and its aglycone, diosgenin, both suppressed the time-dependent increase of blood triacylglycerol level when orally injected with corn oil to mice, suggesting their inhibitory potential against fat absorption. Sprague-Dawley rats fed on a high-fat diet containing 5% Dioscorea nipponica Makino and 40% beef tallow gained significantly less body weight and adipose tissue than control animals fed on a high-fat diet alone during an 8-week experimental period (P<0.05).
AuthorsChong-Suk Kwon, Ho Yong Sohn, Sung Hee Kim, Ji Hyun Kim, Kun Ho Son, Jeong Soon Lee, Jin Kyu Lim, Jong-Sang Kim
JournalBioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 67 Issue 7 Pg. 1451-6 (Jul 2003) ISSN: 0916-8451 [Print] Japan
PMID12913286 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Obesity Agents
  • Dietary Fats
  • Emulsions
  • Plant Extracts
  • Triglycerides
  • dioscin
  • Lipase
  • Diosgenin
  • Adipose Tissue (drug effects)
  • Animals
  • Anti-Obesity Agents (pharmacology, therapeutic use)
  • Body Weight (drug effects)
  • Diet
  • Dietary Fats (administration & dosage, adverse effects)
  • Dioscorea (chemistry)
  • Diosgenin (analogs & derivatives, pharmacology)
  • Emulsions (administration & dosage)
  • Feces (chemistry)
  • Lipase (antagonists & inhibitors)
  • Male
  • Mice
  • Obesity (blood, drug therapy, prevention & control)
  • Organ Size (drug effects)
  • Pancreas (enzymology)
  • Plant Extracts (pharmacology, therapeutic use)
  • Rats
  • Rats, Sprague-Dawley
  • Triglycerides (blood)

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