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[The expression of A103 and inhibin alpha in adrenocortical adenoma by high-throughput tissue microarray techniques].

AbstractOBJECTIVE:
To evaluate the potential diagnostic role of A103 and inhibin alpha in adrenocortical adenoma and the feasibility of tissue microarray/tissue chips.
METHODS:
A series of adrenal gland tissue chips were prepared, including 79 cases of formalin-fixed, paraffin-embedded tissue from 3 normal adrenal glands, 66 adrenocortical adenomas, 5 cases of metastatic carcinoma and 5 pheochromocytomas. A103 and inhibin alpha were detected with Envision immunohistochemical staining methods.
RESULTS:
Positive staining was seen in 3 of 3 (100%) adrenal cortices and in 55 of 61(90.2%) adrenocortical adenomas but in none of the pheochromocytomas or adrenal metastatic carcinomas. In the 3 of 3(100%) adrenal cortices, inhibin alpha immunoreactivity was limited to the zona reticularis and the innermost zona fasciculata. 51 of 61(83.6%) adrenocortical adenomas reacted with inhibin alpha. None of the pheochromocytomas or metastatic carcinomas reacted with inhibin alpha.
CONCLUSIONS:
High-throughput tissue microarray/tissue chip offers a powerful strategy for rapid identification in situ. It is feasible to utilize tissue microarray for a rapid, economic and accurate screening of tissue specimens on a large scale. The combined use of A103 and inhibin alpha is valuable in distinguishing adrenocortical adenoma from pheochromocytoma and other metastatic carcinomas.
AuthorsHongying Zhang, Weiping Liu, Xiaojie Wang, Gandi Li, Jia Guo, Fengyuan Li, Dianying Liao
JournalSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 34 Issue 3 Pg. 424-6 (Jul 2003) ISSN: 1672-173X [Print] China
PMID12910679 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • MART-1 Antigen
  • MLANA protein, human
  • Neoplasm Proteins
  • inhibin-alpha subunit
  • Inhibins
Topics
  • Adrenal Cortex (metabolism)
  • Adrenal Cortex Neoplasms (metabolism)
  • Adrenocortical Adenoma (metabolism)
  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Humans
  • Inhibins (biosynthesis, genetics)
  • MART-1 Antigen
  • Neoplasm Proteins (biosynthesis, genetics)
  • Oligonucleotide Array Sequence Analysis

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